Spectrophotometric Determination of Micro Amounts of Albumin Using Zirconium(IV) and Xylenol Orange

Spectrophotometric Determination of Micro Amounts of Albumin Using Zirconium(IV) and Xylenol Orange

A highly sensitive and selective spectrophotometric method is developed to measure micro amounts of albumin. The proposed method is based on the fact that zirconium(IV)–Xylenol Orange complex fades in the presence of albumin. In the concentration range of 0.1–0.9 µg cm−3, human serum albumin obeys B...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Chemistry letters 2012, Vol.41 (1), p.58-59
Hauptverfasser: Miyachi, Kanako, Kurokawa, Hisashi, Moriyama, Kenzo, Morimoto, Shigefumi, Tabuse, Katsuyoshi, Asano, Mamiko, Yamaguchi, Takako, Fujita, Yoshikazu
Format: Artikel
Sprache:eng
Schlagworte:
Absorptivity
Albumins
Human
Serum albumin
Spectrophotometry
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:A highly sensitive and selective spectrophotometric method is developed to measure micro amounts of albumin. The proposed method is based on the fact that zirconium(IV)–Xylenol Orange complex fades in the presence of albumin. In the concentration range of 0.1–0.9 µg cm−3, human serum albumin obeys Beer’s law and shows an effective molar absorptivity of 8.4 × 107 dm3 mol−1 cm−1 at 605 nm.
ISSN:0366-7022
1348-0715
DOI:10.1246/cl.2012.58