Paper diagnostic device for quantitative electrochemical detection of ricin at picomolar levels

We report a paper-based assay platform for detection of ricin a chain. The paper platform is assembled by simple origami paper folding. The sensor is based on quantitative, electrochemical detection of silver nanoparticle labels linked to a magnetic microbead support via a ricin immunosandwich. Impo...

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Veröffentlicht in:Lab on a chip 2015-01, Vol.15 (18), p.3707-3715
Hauptverfasser: Cunningham, Josephine C, Scida, Karen, Kogan, Molly R, Wang, Bo, Ellington, Andrew D, Crooks, Richard M
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Sprache:eng
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Zusammenfassung:We report a paper-based assay platform for detection of ricin a chain. The paper platform is assembled by simple origami paper folding. The sensor is based on quantitative, electrochemical detection of silver nanoparticle labels linked to a magnetic microbead support via a ricin immunosandwich. Importantly, ricin was detected at concentrations as low as 34 pM. Additionally, the assay is robust, even in the presence of 100-fold excess hoax materials. Finally, the device is easily remediated after use by incineration. The cost of the device, not including reagents, is just $0.30. The total assay time, including formation of the immunosandwich, is 9.5 min.
ISSN:1473-0197
1473-0189
DOI:10.1039/c5lc00731c