Pulsed EPR spectroscopy distance measurements of DNA internally labelled with Gd super(3+)-DOTA

Pulsed EPR spectroscopy distance measurements of DNA internally labelled with Gd super(3+)-DOTA

Gd super(3+) is increasingly used in EPR spectroscopy due to its increased intracellular stability and signal-to-noise ratios. Here we present the incorporation of Gd super(3+)-DOTA into internal positions in DNA. Distance measurements via pulsed Electron Paramagnetic Resonance (EPR) spectroscopy in...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2015-08, Vol.51 (72), p.13850-13853
Hauptverfasser: Wojciechowski, Filip, Gros, Andreas, Holder, Isabelle T, Knorr, Laura, Drescher, Malte, Hartig, Jorg S
Format: Artikel
Sprache:eng
Schlagworte:
Deoxyribonucleic acid
Electron paramagnetic resonance
Labels
Signal to noise ratio
Spectroscopy
Stability
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Zusammenfassung:Gd super(3+) is increasingly used in EPR spectroscopy due to its increased intracellular stability and signal-to-noise ratios. Here we present the incorporation of Gd super(3+)-DOTA into internal positions in DNA. Distance measurements via pulsed Electron Paramagnetic Resonance (EPR) spectroscopy in vitro and in cellula proved enhanced stability and efficiency compared to nitroxide labels.
ISSN:1359-7345
1364-548X
DOI:10.1039/c5cc04234h