Specific Amino Acid Sensing Using a Single Acridone-labeled DNA Aptamer

Specific Amino Acid Sensing Using a Single Acridone-labeled DNA Aptamer

Acridone is an attractive fluorescent molecule for use in DNA probes. Sensing molecule, which has acridone as a signaling moiety and DNA aptamer as a recognition moiety, was prepared by postsynthetic modification method. The fluorescence of this sensing molecule changed depending on the DNA structur...

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Veröffentlicht in:Chemistry letters 2012-09, Vol.41 (9), p.917-919
Hauptverfasser: Ozaki, Hiroaki, Hagiwara, Yasuhisa, Kuwahara, Masayasu
Format: Artikel
Sprache:eng
Schlagworte:
Amino acids
Beacons
Deoxyribonucleic acid
Detection
Fluorescence
Recognition
Strategy
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Zusammenfassung:Acridone is an attractive fluorescent molecule for use in DNA probes. Sensing molecule, which has acridone as a signaling moiety and DNA aptamer as a recognition moiety, was prepared by postsynthetic modification method. The fluorescence of this sensing molecule changed depending on the DNA structure and L-argininamide could be detected by this molecule. This strategy could provide a quencher-free aptamer beacon.
ISSN:0366-7022
1348-0715
DOI:10.1246/cl.2012.917