Detection of transgenerational barium dual-isotope marks in salmon otoliths by means of LA-ICP-MS
The present study evaluates the use of an individual-specific transgenerational barium dual-isotope procedure and its application to salmon specimens from the Sella River (Asturias, Spain). For such a purpose, the use of laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) in comb...
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Veröffentlicht in: | Analytical and bioanalytical chemistry 2013-03, Vol.405 (9), p.2901-2909 |
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Sprache: | eng |
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Zusammenfassung: | The present study evaluates the use of an individual-specific transgenerational barium dual-isotope procedure and its application to salmon specimens from the Sella River (Asturias, Spain). For such a purpose, the use of laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) in combination with multiple linear regression for the determination of the isotopic mark in the otoliths of the specimens is presented. In this sense, a solution in which two barium-enriched isotopes (
137
Ba and
135
Ba) were mixed at a molar ratio of ca. 1:3 (
N
Ba137
/
N
Ba135
) was administered to eight returning females caught during the spawning period. After injection, these females, as well as their offspring, were reared in a governmental hatchery located in the council of Cangas de Onís (Asturias, Spain). For comparison purposes, as well as for a time-monitoring control, egg and larva data obtained by solution analysis ICP-MS are also given. Otoliths (9-month-old juveniles) of marked offspring were analysed by LA-ICP-MS demonstrating a 100 % marking efficacy of this methodology. The capabilities of the molar fraction approach for 2D imaging of fish otoliths are also addressed. |
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ISSN: | 1618-2642 1618-2650 |
DOI: | 10.1007/s00216-012-6452-2 |