Effect of acute pancreatitis on the pharmacokinetics of Chinese herbal micron Liuhe Pill ointment (微米六合丹) in rats
Objective To explore the effect of acute pancreatitis (AP) on the pharmacokinetics of herbal ointment micron Liuhe Pill (微米六合丹, MLHP) components in anesthetized rats. Methods Rats were randomly divided into a AP model group ( n =6) and a normal group as a control ( n =6). The rat model of AP was ind...
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| Veröffentlicht in: | Chinese journal of integrative medicine 2015-12, Vol.21 (12), p.922-927 |
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| creator | Liu, Yi-ling Zhao, Xian-lin Li, Juan Wan, Mei-hua Chen, Guang-yuan Chen, Wei-wei Tang, Wen-fu |
| description | Objective
To explore the effect of acute pancreatitis (AP) on the pharmacokinetics of herbal ointment micron Liuhe Pill (微米六合丹, MLHP) components in anesthetized rats.
Methods
Rats were randomly divided into a AP model group (
n
=6) and a normal group as a control (
n
=6). The rat model of AP was induced by intraperitoneal injection of L-arginine in rats (15 mg/kg, twice, interval 1 h). Chinese herbal ointment MLHP was used externally on the belly after the 2nd injection for 48 h in both groups. Emodin, rhein, aloe emodin, physcion, chrysophanol from MLHP were detected and quantified in rat serum and pancreas (at 48 h) by high performance liquid chromatography-tandem mass spectrometry.
Results
Among the five components, only emodin, aloe emodin and physcion from MLHP were detected in all rat serum and most of the rats' pancreas. Rhein and chrysophanol were not detected in both serum and pancreas. T
1/2α
of emodin and physcion in MLHP were obviously shorter in the AP model group than those in the normal group (
P |
| doi_str_mv | 10.1007/s11655-015-2080-y |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1744660145</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1744660145</sourcerecordid><originalsourceid>FETCH-LOGICAL-c414t-93f320a8252923ab21ea3d32603fc925bd2369e779bb4b9e6f0b3cbcc324c6db3</originalsourceid><addsrcrecordid>eNp9kL9uFDEQhy0EIn8fgAa5TIqFsb323pbolADSSVBAbdm-Wc5h13vY3uKUGomSFwEJpSFNnoYTr4FPFyipZsb-5ifNR8gTBs8YQPM8MaakrIDJisMMqs0DcsjaVlRQA39YetXw0jN5QI5SugKQjQL5mBxwxcRMCDgk1xddhy7TsaPGTRnp2gQX0WSffaJjoHlV3lYmDsaNH33A7F3a0fNVGRLSFUZrejp4Fwu98FPh3_q-p6MPecCQ6dn27vvvHzfbz9-2X7_8-nl7Tn2g0eR0Qh51pk94el-PyfvLi3fzV9XizcvX8xeLytWszlUrOsHBzLjkLRfGcoZGLAVXIDrXcmmXXKgWm6a1trYtqg6scNY5wWunllYck7N97jqOnyZMWQ8-Oex7E3CckmZNXSsFrJYFZXu0XJNSxE6vox9M3GgGeudc753r4lzvnOtN2Xl6Hz_ZAZf_Nv5KLgDfA6l8hQ8Y9dU4xVBO_k_qHxfmkzc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1744660145</pqid></control><display><type>article</type><title>Effect of acute pancreatitis on the pharmacokinetics of Chinese herbal micron Liuhe Pill ointment (微米六合丹) in rats</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><source>Alma/SFX Local Collection</source><creator>Liu, Yi-ling ; Zhao, Xian-lin ; Li, Juan ; Wan, Mei-hua ; Chen, Guang-yuan ; Chen, Wei-wei ; Tang, Wen-fu</creator><creatorcontrib>Liu, Yi-ling ; Zhao, Xian-lin ; Li, Juan ; Wan, Mei-hua ; Chen, Guang-yuan ; Chen, Wei-wei ; Tang, Wen-fu</creatorcontrib><description>Objective
To explore the effect of acute pancreatitis (AP) on the pharmacokinetics of herbal ointment micron Liuhe Pill (微米六合丹, MLHP) components in anesthetized rats.
Methods
Rats were randomly divided into a AP model group (
n
=6) and a normal group as a control (
n
=6). The rat model of AP was induced by intraperitoneal injection of L-arginine in rats (15 mg/kg, twice, interval 1 h). Chinese herbal ointment MLHP was used externally on the belly after the 2nd injection for 48 h in both groups. Emodin, rhein, aloe emodin, physcion, chrysophanol from MLHP were detected and quantified in rat serum and pancreas (at 48 h) by high performance liquid chromatography-tandem mass spectrometry.
Results
Among the five components, only emodin, aloe emodin and physcion from MLHP were detected in all rat serum and most of the rats' pancreas. Rhein and chrysophanol were not detected in both serum and pancreas. T
1/2α
of emodin and physcion in MLHP were obviously shorter in the AP model group than those in the normal group (
P
<0.05), while there was no difference for T
1/2α
of aloe emodin. The peak concentration and area under curve of all three components were much higher in the AP group than those in the normal group with MLHP in external application for 48 h (
P
<0.05). Furthermore, the mean residence time (MRT) and maximum plasma concentration (Tmax) of emodin and aloe emodin were obviously longer in the AP model group than those in the normal control group (
P
<0.05). There was no significant difference for Ka of all components between the two groups. Emodin could be detected in all rats' pancreas at 48 h in both groups, while its mean pancreatic concentration was higher in the AP model group than in the normal group (0.61±0.54 ng/mL, 0.42±0.37 ng/mL, respectively,
P
<0.05). Aloe emodin could be detected in all rats' pancreas at 48 h in both groups and their mean pancreatic concentration were similar (0.31±0.24 ng/mL, 0.33±0.17 ng/mL, respectively,
P
>0.05). Physcion could be detected in pancreas of most rats in the AP model while only two rats in the normal group.
Conclusion
AP could significantly affect the pharmacokinetics of absorbed components of Chinese herbal MLHP ointment in rats.</description><identifier>ISSN: 1672-0415</identifier><identifier>EISSN: 1993-0402</identifier><identifier>DOI: 10.1007/s11655-015-2080-y</identifier><identifier>PMID: 26138330</identifier><language>eng</language><publisher>Beijing: Chinese Association of Traditional and Western Medicine</publisher><subject>Acute Disease ; Animals ; Anthraquinones - analysis ; Drugs, Chinese Herbal - analysis ; Drugs, Chinese Herbal - pharmacokinetics ; Emodin - analysis ; Male ; Medicine ; Medicine & Public Health ; Ointments ; Original Article ; Pancreatitis - metabolism ; Rats ; Rats, Sprague-Dawley</subject><ispartof>Chinese journal of integrative medicine, 2015-12, Vol.21 (12), p.922-927</ispartof><rights>Chinese Association of the Integration of Traditional and Western Medicine and Springer-Verlag Berlin Heidelberg 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c414t-93f320a8252923ab21ea3d32603fc925bd2369e779bb4b9e6f0b3cbcc324c6db3</citedby><cites>FETCH-LOGICAL-c414t-93f320a8252923ab21ea3d32603fc925bd2369e779bb4b9e6f0b3cbcc324c6db3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11655-015-2080-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11655-015-2080-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,778,782,27911,27912,41475,42544,51306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26138330$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Yi-ling</creatorcontrib><creatorcontrib>Zhao, Xian-lin</creatorcontrib><creatorcontrib>Li, Juan</creatorcontrib><creatorcontrib>Wan, Mei-hua</creatorcontrib><creatorcontrib>Chen, Guang-yuan</creatorcontrib><creatorcontrib>Chen, Wei-wei</creatorcontrib><creatorcontrib>Tang, Wen-fu</creatorcontrib><title>Effect of acute pancreatitis on the pharmacokinetics of Chinese herbal micron Liuhe Pill ointment (微米六合丹) in rats</title><title>Chinese journal of integrative medicine</title><addtitle>Chin. J. Integr. Med</addtitle><addtitle>Chin J Integr Med</addtitle><description>Objective
To explore the effect of acute pancreatitis (AP) on the pharmacokinetics of herbal ointment micron Liuhe Pill (微米六合丹, MLHP) components in anesthetized rats.
Methods
Rats were randomly divided into a AP model group (
n
=6) and a normal group as a control (
n
=6). The rat model of AP was induced by intraperitoneal injection of L-arginine in rats (15 mg/kg, twice, interval 1 h). Chinese herbal ointment MLHP was used externally on the belly after the 2nd injection for 48 h in both groups. Emodin, rhein, aloe emodin, physcion, chrysophanol from MLHP were detected and quantified in rat serum and pancreas (at 48 h) by high performance liquid chromatography-tandem mass spectrometry.
Results
Among the five components, only emodin, aloe emodin and physcion from MLHP were detected in all rat serum and most of the rats' pancreas. Rhein and chrysophanol were not detected in both serum and pancreas. T
1/2α
of emodin and physcion in MLHP were obviously shorter in the AP model group than those in the normal group (
P
<0.05), while there was no difference for T
1/2α
of aloe emodin. The peak concentration and area under curve of all three components were much higher in the AP group than those in the normal group with MLHP in external application for 48 h (
P
<0.05). Furthermore, the mean residence time (MRT) and maximum plasma concentration (Tmax) of emodin and aloe emodin were obviously longer in the AP model group than those in the normal control group (
P
<0.05). There was no significant difference for Ka of all components between the two groups. Emodin could be detected in all rats' pancreas at 48 h in both groups, while its mean pancreatic concentration was higher in the AP model group than in the normal group (0.61±0.54 ng/mL, 0.42±0.37 ng/mL, respectively,
P
<0.05). Aloe emodin could be detected in all rats' pancreas at 48 h in both groups and their mean pancreatic concentration were similar (0.31±0.24 ng/mL, 0.33±0.17 ng/mL, respectively,
P
>0.05). Physcion could be detected in pancreas of most rats in the AP model while only two rats in the normal group.
Conclusion
AP could significantly affect the pharmacokinetics of absorbed components of Chinese herbal MLHP ointment in rats.</description><subject>Acute Disease</subject><subject>Animals</subject><subject>Anthraquinones - analysis</subject><subject>Drugs, Chinese Herbal - analysis</subject><subject>Drugs, Chinese Herbal - pharmacokinetics</subject><subject>Emodin - analysis</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Ointments</subject><subject>Original Article</subject><subject>Pancreatitis - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><issn>1672-0415</issn><issn>1993-0402</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kL9uFDEQhy0EIn8fgAa5TIqFsb323pbolADSSVBAbdm-Wc5h13vY3uKUGomSFwEJpSFNnoYTr4FPFyipZsb-5ifNR8gTBs8YQPM8MaakrIDJisMMqs0DcsjaVlRQA39YetXw0jN5QI5SugKQjQL5mBxwxcRMCDgk1xddhy7TsaPGTRnp2gQX0WSffaJjoHlV3lYmDsaNH33A7F3a0fNVGRLSFUZrejp4Fwu98FPh3_q-p6MPecCQ6dn27vvvHzfbz9-2X7_8-nl7Tn2g0eR0Qh51pk94el-PyfvLi3fzV9XizcvX8xeLytWszlUrOsHBzLjkLRfGcoZGLAVXIDrXcmmXXKgWm6a1trYtqg6scNY5wWunllYck7N97jqOnyZMWQ8-Oex7E3CckmZNXSsFrJYFZXu0XJNSxE6vox9M3GgGeudc753r4lzvnOtN2Xl6Hz_ZAZf_Nv5KLgDfA6l8hQ8Y9dU4xVBO_k_qHxfmkzc</recordid><startdate>20151201</startdate><enddate>20151201</enddate><creator>Liu, Yi-ling</creator><creator>Zhao, Xian-lin</creator><creator>Li, Juan</creator><creator>Wan, Mei-hua</creator><creator>Chen, Guang-yuan</creator><creator>Chen, Wei-wei</creator><creator>Tang, Wen-fu</creator><general>Chinese Association of Traditional and Western Medicine</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20151201</creationdate><title>Effect of acute pancreatitis on the pharmacokinetics of Chinese herbal micron Liuhe Pill ointment (微米六合丹) in rats</title><author>Liu, Yi-ling ; Zhao, Xian-lin ; Li, Juan ; Wan, Mei-hua ; Chen, Guang-yuan ; Chen, Wei-wei ; Tang, Wen-fu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c414t-93f320a8252923ab21ea3d32603fc925bd2369e779bb4b9e6f0b3cbcc324c6db3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Acute Disease</topic><topic>Animals</topic><topic>Anthraquinones - analysis</topic><topic>Drugs, Chinese Herbal - analysis</topic><topic>Drugs, Chinese Herbal - pharmacokinetics</topic><topic>Emodin - analysis</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Ointments</topic><topic>Original Article</topic><topic>Pancreatitis - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Yi-ling</creatorcontrib><creatorcontrib>Zhao, Xian-lin</creatorcontrib><creatorcontrib>Li, Juan</creatorcontrib><creatorcontrib>Wan, Mei-hua</creatorcontrib><creatorcontrib>Chen, Guang-yuan</creatorcontrib><creatorcontrib>Chen, Wei-wei</creatorcontrib><creatorcontrib>Tang, Wen-fu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Chinese journal of integrative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Yi-ling</au><au>Zhao, Xian-lin</au><au>Li, Juan</au><au>Wan, Mei-hua</au><au>Chen, Guang-yuan</au><au>Chen, Wei-wei</au><au>Tang, Wen-fu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of acute pancreatitis on the pharmacokinetics of Chinese herbal micron Liuhe Pill ointment (微米六合丹) in rats</atitle><jtitle>Chinese journal of integrative medicine</jtitle><stitle>Chin. J. Integr. Med</stitle><addtitle>Chin J Integr Med</addtitle><date>2015-12-01</date><risdate>2015</risdate><volume>21</volume><issue>12</issue><spage>922</spage><epage>927</epage><pages>922-927</pages><issn>1672-0415</issn><eissn>1993-0402</eissn><abstract>Objective
To explore the effect of acute pancreatitis (AP) on the pharmacokinetics of herbal ointment micron Liuhe Pill (微米六合丹, MLHP) components in anesthetized rats.
Methods
Rats were randomly divided into a AP model group (
n
=6) and a normal group as a control (
n
=6). The rat model of AP was induced by intraperitoneal injection of L-arginine in rats (15 mg/kg, twice, interval 1 h). Chinese herbal ointment MLHP was used externally on the belly after the 2nd injection for 48 h in both groups. Emodin, rhein, aloe emodin, physcion, chrysophanol from MLHP were detected and quantified in rat serum and pancreas (at 48 h) by high performance liquid chromatography-tandem mass spectrometry.
Results
Among the five components, only emodin, aloe emodin and physcion from MLHP were detected in all rat serum and most of the rats' pancreas. Rhein and chrysophanol were not detected in both serum and pancreas. T
1/2α
of emodin and physcion in MLHP were obviously shorter in the AP model group than those in the normal group (
P
<0.05), while there was no difference for T
1/2α
of aloe emodin. The peak concentration and area under curve of all three components were much higher in the AP group than those in the normal group with MLHP in external application for 48 h (
P
<0.05). Furthermore, the mean residence time (MRT) and maximum plasma concentration (Tmax) of emodin and aloe emodin were obviously longer in the AP model group than those in the normal control group (
P
<0.05). There was no significant difference for Ka of all components between the two groups. Emodin could be detected in all rats' pancreas at 48 h in both groups, while its mean pancreatic concentration was higher in the AP model group than in the normal group (0.61±0.54 ng/mL, 0.42±0.37 ng/mL, respectively,
P
<0.05). Aloe emodin could be detected in all rats' pancreas at 48 h in both groups and their mean pancreatic concentration were similar (0.31±0.24 ng/mL, 0.33±0.17 ng/mL, respectively,
P
>0.05). Physcion could be detected in pancreas of most rats in the AP model while only two rats in the normal group.
Conclusion
AP could significantly affect the pharmacokinetics of absorbed components of Chinese herbal MLHP ointment in rats.</abstract><cop>Beijing</cop><pub>Chinese Association of Traditional and Western Medicine</pub><pmid>26138330</pmid><doi>10.1007/s11655-015-2080-y</doi><tpages>6</tpages></addata></record> |
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| ispartof | Chinese journal of integrative medicine, 2015-12, Vol.21 (12), p.922-927 |
| issn | 1672-0415 1993-0402 |
| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals; Alma/SFX Local Collection |
| subjects | Acute Disease Animals Anthraquinones - analysis Drugs, Chinese Herbal - analysis Drugs, Chinese Herbal - pharmacokinetics Emodin - analysis Male Medicine Medicine & Public Health Ointments Original Article Pancreatitis - metabolism Rats Rats, Sprague-Dawley |
| title | Effect of acute pancreatitis on the pharmacokinetics of Chinese herbal micron Liuhe Pill ointment (微米六合丹) in rats |
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