Effect of acute pancreatitis on the pharmacokinetics of Chinese herbal micron Liuhe Pill ointment (微米六合丹) in rats

Objective To explore the effect of acute pancreatitis (AP) on the pharmacokinetics of herbal ointment micron Liuhe Pill (微米六合丹, MLHP) components in anesthetized rats. Methods Rats were randomly divided into a AP model group ( n =6) and a normal group as a control ( n =6). The rat model of AP was induced by intraperitoneal injection of L-arginine in rats (15 mg/kg, twice, interval 1 h). Chinese herbal ointment MLHP was used externally on the belly after the 2nd injection for 48 h in both groups. Emodin, rhein, aloe emodin, physcion, chrysophanol from MLHP were detected and quantified in rat serum and pancreas (at 48 h) by high performance liquid chromatography-tandem mass spectrometry. Results Among the five components, only emodin, aloe emodin and physcion from MLHP were detected in all rat serum and most of the rats' pancreas. Rhein and chrysophanol were not detected in both serum and pancreas. T 1/2α of emodin and physcion in MLHP were obviously shorter in the AP model group than those in the normal group ( P