Isolation and characterization of microsatellite loci from Psidium guajava L

A (GA)n and (GT)n microsatellite‐enriched library was constructed and 23 nuclear simple sequence repeat (SSR) loci were characterized in the guava species (Psidium guajava L.). All SSR loci were found to be polymorphic after screening for diversity in different cultivars, and across‐taxa amplificati...

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Veröffentlicht in:Molecular ecology notes 2005-12, Vol.5 (4), p.745-748
Hauptverfasser: Risterucci, A.M, Duval, M.F, Rohde, W, Billotte, N
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Sprache:eng
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Zusammenfassung:A (GA)n and (GT)n microsatellite‐enriched library was constructed and 23 nuclear simple sequence repeat (SSR) loci were characterized in the guava species (Psidium guajava L.). All SSR loci were found to be polymorphic after screening for diversity in different cultivars, and across‐taxa amplification tests showed the potential transferability of most SSR markers in three other Psidium species. First to be published for P. guajava, this new SSR resource will be a powerful tool for genetic studies of guava, including cultivars identification and linkage mapping, as well as potentially for interspecific genetic studies within the genus Psidium.
ISSN:1471-8278
1471-8286
DOI:10.1111/j.1471-8286.2005.01050.x