Separation and identification of antioxidative peptides from peptic digest of fish scale collagen

Separation and identification of antioxidative peptides from peptic digest of fish scale collagen

A peptidic fraction having antioxidant activity was separated from the peptic digest of mackerel pike scale collagen by ion-exchange, gel-filtration chromatography, and HPLC. Peptides obatained by SP-Sephadex C-25 chromatography of the most active fraction (SP-III) were further separated using ODS H...

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Veröffentlicht in:Journal of National Fisheries University (Japan) 2004-02, Vol.52 (2), p.57-62
Hauptverfasser: Suetsuna, K. (National Fisheries Univ., Shimonoseki, Yamaguchi (Japan)), Maekawa, K, Chen, J.R, Harada, K, Hamada, M, Shin, S.U
Format: Artikel
Sprache:jpn
Schlagworte:
ANTIOXIDANTS
COLOLABIS
IDENTIFICATION
PEPSIN
PEPTIDES
SCALES
SEPARATING
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Zusammenfassung:A peptidic fraction having antioxidant activity was separated from the peptic digest of mackerel pike scale collagen by ion-exchange, gel-filtration chromatography, and HPLC. Peptides obatained by SP-Sephadex C-25 chromatography of the most active fraction (SP-III) were further separated using ODS HPLC. The amino acid sequences of these peptides were Leu-His-Gln-Pro-Val-Pro-Glu (LHQPVPE) and Val-Ser-Gln-Pro-Ile-Gln-Gln-Glu (VSQPIQQE). The IC50 values of LHQPVPE and VSQPIQQE for hydroxyl radical scavenging activity were 27.3 micro M and 7.53 micro M, respectively.
ISSN:0370-9361