A method to size DNA fragments from 50 to 800 bp on a DNA analyser

A method to size DNA fragments from 50 to 800 bp on a DNA analyser

We present a method to size DNA fragments from 50 to 800 bp on a DNA analyser using an alternative size standard (X‐Rhodamine MapMarker 1000) run on a capillary electrophoresis system (Applied Biosystem's 3730 DNA Analyser). This method avoids several limitations imposed by manufacturer‐develop...

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Veröffentlicht in:Molecular ecology notes 2005-12, Vol.5 (4), p.969-970
Hauptverfasser: AVIS, PETER G., FELDHEIM, KEVIN A.
Format: Artikel
Sprache:eng
Schlagworte:
capillary electrophoresis
fluorescently labelled size standard
fragment analysis
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Beschreibung
Zusammenfassung:We present a method to size DNA fragments from 50 to 800 bp on a DNA analyser using an alternative size standard (X‐Rhodamine MapMarker 1000) run on a capillary electrophoresis system (Applied Biosystem's 3730 DNA Analyser). This method avoids several limitations imposed by manufacturer‐developed size standards, instruments and software and offers a more robust approach to conducting DNA size fragment analyses.
ISSN:1471-8278
1471-8286
DOI:10.1111/j.1471-8286.2005.01105.x