Fabrication and Endothelialization of Collagen-Blended Biodegradable Polymer Nanofibers: Potential Vascular Graft for Blood Vessel Tissue Engineering
Electrospun collagen-blended poly(L-lactic acid)- co -poly(∈-caprolactone) [P(LLA-CL), 70:30] nanofiber may have great potential application in tissue engineering because it mimicks the extracellular matrix (ECM) both morphologically and chemically. Blended nanofibers with various weight ratios of p...
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| Veröffentlicht in: | Tissue engineering 2005-09, Vol.11 (9-10), p.1574-1588 |
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| container_title | Tissue engineering |
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| creator | He, Wei Yong, Thomas Teo, Wee Eong Ma, Zuwei Ramakrishna, Seeram |
| description | Electrospun collagen-blended poly(L-lactic acid)-
co
-poly(∈-caprolactone) [P(LLA-CL), 70:30]
nanofiber may have great potential application in tissue engineering because it mimicks the extracellular
matrix (ECM) both morphologically and chemically. Blended nanofibers with various weight
ratios of polymer to collagen were fabricated by electrospinning. The appearance of the blended
nanofibers was investigated by scanning electron microscopy and transmission electron microscopy.
The nanofibers exhibited a smooth surface and a narrow diameter distribution, with 60% of the
nanofibers having diameters between 100 and 200 nm. Attenuated total reflectance-Fourier transform
infrared spectra and X-ray photoelectron spectroscopy verified the existence of collagen molecules
on the surface of nanofibers. Human coronary artery endothelial cells (HCAECs) were seeded
onto the blended nanofibers for viability, morphogenesis, attachment, and phenotypic studies. Five
characteristic endothelial cell (EC) markers, including four types of cell adhesion molecule and one
EC-preferential gene (von Willebrand factor), were studied by reverse transcription-polymerase
chain reaction. Results showed that the collagen-blended polymer nanofibers could enhance the viability,
spreading, and attachment of HCAECs and, moreover, preserve the EC phenotype. The
blending electrospinning technique shows potential in refining the composition of polymer nanofibers
by adding various ingredients (e.g., growth factors) according to cell types to fabricate tissue-engineering
scaffold, particularly blood vessel-engineering scaffold. |
| doi_str_mv | 10.1089/ten.2005.11.1574 |
| format | Article |
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co
-poly(∈-caprolactone) [P(LLA-CL), 70:30]
nanofiber may have great potential application in tissue engineering because it mimicks the extracellular
matrix (ECM) both morphologically and chemically. Blended nanofibers with various weight
ratios of polymer to collagen were fabricated by electrospinning. The appearance of the blended
nanofibers was investigated by scanning electron microscopy and transmission electron microscopy.
The nanofibers exhibited a smooth surface and a narrow diameter distribution, with 60% of the
nanofibers having diameters between 100 and 200 nm. Attenuated total reflectance-Fourier transform
infrared spectra and X-ray photoelectron spectroscopy verified the existence of collagen molecules
on the surface of nanofibers. Human coronary artery endothelial cells (HCAECs) were seeded
onto the blended nanofibers for viability, morphogenesis, attachment, and phenotypic studies. Five
characteristic endothelial cell (EC) markers, including four types of cell adhesion molecule and one
EC-preferential gene (von Willebrand factor), were studied by reverse transcription-polymerase
chain reaction. Results showed that the collagen-blended polymer nanofibers could enhance the viability,
spreading, and attachment of HCAECs and, moreover, preserve the EC phenotype. The
blending electrospinning technique shows potential in refining the composition of polymer nanofibers
by adding various ingredients (e.g., growth factors) according to cell types to fabricate tissue-engineering
scaffold, particularly blood vessel-engineering scaffold.</description><identifier>ISSN: 1076-3279</identifier><identifier>EISSN: 1557-8690</identifier><identifier>DOI: 10.1089/ten.2005.11.1574</identifier><identifier>PMID: 16259611</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Biocompatible Materials - chemical synthesis ; Biocompatible Materials - chemistry ; Biodegradable materials ; Biodegradation, Environmental ; Biomimetic Materials - chemical synthesis ; Biomimetic Materials - chemistry ; Blood Vessel Prosthesis ; Cell Adhesion ; Cell Adhesion Molecules - metabolism ; Cell Culture Techniques ; Cell Survival ; Cells, Cultured ; Collagen - metabolism ; Coronary Vessels - cytology ; Culture Media - chemistry ; Culture Media - pharmacology ; Endothelium, Vascular - cytology ; Endothelium, Vascular - drug effects ; Endothelium, Vascular - metabolism ; Endothelium, Vascular - physiology ; Endothelium, Vascular - ultrastructure ; Gene Expression ; Humans ; Materials Testing ; Nanotechnology ; Polyesters - chemical synthesis ; Polyesters - chemistry ; Polymers ; Reverse Transcriptase Polymerase Chain Reaction ; Skin & tissue grafts ; Spectrometry, X-Ray Emission ; Spectroscopy, Fourier Transform Infrared ; Tensile Strength ; Time Factors ; Tissue engineering ; Tissue Engineering - methods</subject><ispartof>Tissue engineering, 2005-09, Vol.11 (9-10), p.1574-1588</ispartof><rights>2005, Mary Ann Liebert, Inc.</rights><rights>(©) Copyright 2005, Mary Ann Liebert, Inc.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c460t-53db25064002fdd048a34408fa0ff7876ae813ff112444615f4afd912c69e6273</citedby><cites>FETCH-LOGICAL-c460t-53db25064002fdd048a34408fa0ff7876ae813ff112444615f4afd912c69e6273</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.liebertpub.com/doi/epdf/10.1089/ten.2005.11.1574$$EPDF$$P50$$Gmaryannliebert$$H</linktopdf><linktohtml>$$Uhttps://www.liebertpub.com/doi/full/10.1089/ten.2005.11.1574$$EHTML$$P50$$Gmaryannliebert$$H</linktohtml><link.rule.ids>314,780,784,3042,21723,27924,27925,55291,55303</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16259611$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>He, Wei</creatorcontrib><creatorcontrib>Yong, Thomas</creatorcontrib><creatorcontrib>Teo, Wee Eong</creatorcontrib><creatorcontrib>Ma, Zuwei</creatorcontrib><creatorcontrib>Ramakrishna, Seeram</creatorcontrib><title>Fabrication and Endothelialization of Collagen-Blended Biodegradable Polymer Nanofibers: Potential Vascular Graft for Blood Vessel Tissue Engineering</title><title>Tissue engineering</title><addtitle>Tissue Eng</addtitle><description>Electrospun collagen-blended poly(L-lactic acid)-
co
-poly(∈-caprolactone) [P(LLA-CL), 70:30]
nanofiber may have great potential application in tissue engineering because it mimicks the extracellular
matrix (ECM) both morphologically and chemically. Blended nanofibers with various weight
ratios of polymer to collagen were fabricated by electrospinning. The appearance of the blended
nanofibers was investigated by scanning electron microscopy and transmission electron microscopy.
The nanofibers exhibited a smooth surface and a narrow diameter distribution, with 60% of the
nanofibers having diameters between 100 and 200 nm. Attenuated total reflectance-Fourier transform
infrared spectra and X-ray photoelectron spectroscopy verified the existence of collagen molecules
on the surface of nanofibers. Human coronary artery endothelial cells (HCAECs) were seeded
onto the blended nanofibers for viability, morphogenesis, attachment, and phenotypic studies. Five
characteristic endothelial cell (EC) markers, including four types of cell adhesion molecule and one
EC-preferential gene (von Willebrand factor), were studied by reverse transcription-polymerase
chain reaction. Results showed that the collagen-blended polymer nanofibers could enhance the viability,
spreading, and attachment of HCAECs and, moreover, preserve the EC phenotype. The
blending electrospinning technique shows potential in refining the composition of polymer nanofibers
by adding various ingredients (e.g., growth factors) according to cell types to fabricate tissue-engineering
scaffold, particularly blood vessel-engineering scaffold.</description><subject>Biocompatible Materials - chemical synthesis</subject><subject>Biocompatible Materials - chemistry</subject><subject>Biodegradable materials</subject><subject>Biodegradation, Environmental</subject><subject>Biomimetic Materials - chemical synthesis</subject><subject>Biomimetic Materials - chemistry</subject><subject>Blood Vessel Prosthesis</subject><subject>Cell Adhesion</subject><subject>Cell Adhesion Molecules - metabolism</subject><subject>Cell Culture Techniques</subject><subject>Cell Survival</subject><subject>Cells, Cultured</subject><subject>Collagen - metabolism</subject><subject>Coronary Vessels - cytology</subject><subject>Culture Media - chemistry</subject><subject>Culture Media - pharmacology</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - drug effects</subject><subject>Endothelium, Vascular - metabolism</subject><subject>Endothelium, Vascular - physiology</subject><subject>Endothelium, Vascular - ultrastructure</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>Materials Testing</subject><subject>Nanotechnology</subject><subject>Polyesters - chemical synthesis</subject><subject>Polyesters - chemistry</subject><subject>Polymers</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Skin & tissue grafts</subject><subject>Spectrometry, X-Ray Emission</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>Tensile Strength</subject><subject>Time Factors</subject><subject>Tissue engineering</subject><subject>Tissue Engineering - methods</subject><issn>1076-3279</issn><issn>1557-8690</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNplkU9v1DAQxSMEoqVw54QsDr0leBzbSbixq_6TqpZD6dVy4vHiymsXOzm034Pvi1e7Aomexnr6zZvxvKr6CLQB2g9fZgwNo1Q0AA2Ijr-qjkGIru7lQF-XN-1k3bJuOKre5fxACymge1sdgWRikADH1e9zPSY36dnFQHQw5CyYOP9E77R3z3s5WrKO3usNhnrlMRg0ZOWiwU3SRo8eyffon7aYyI0O0boRU_5atLLdXGzIvc7T4nUiF0nbmdiYyMrHaMg95oye3LmcFyyTNy4gJhc276s3VvuMHw71pPpxfna3vqyvby-u1t-u64lLOteiNSMTVHJKmTWG8l63nNPeampt13dSYw-ttQCMcy5BWK6tGYBNckDJuvakOt37Pqb4a8E8q63LE5a_BoxLVtBxRgfgBfz8H_gQlxTKboqBkFC8-gLRPTSlmHNCqx6T2-r0pICqXV6qXETt8lIAapdXafl08F3GLZp_DYeACtDsgZ2sQ_AOy3nnv-ALxz-NjaKi</recordid><startdate>20050901</startdate><enddate>20050901</enddate><creator>He, Wei</creator><creator>Yong, Thomas</creator><creator>Teo, Wee Eong</creator><creator>Ma, Zuwei</creator><creator>Ramakrishna, Seeram</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20050901</creationdate><title>Fabrication and Endothelialization of Collagen-Blended Biodegradable Polymer Nanofibers: Potential Vascular Graft for Blood Vessel Tissue Engineering</title><author>He, Wei ; Yong, Thomas ; Teo, Wee Eong ; Ma, Zuwei ; Ramakrishna, Seeram</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c460t-53db25064002fdd048a34408fa0ff7876ae813ff112444615f4afd912c69e6273</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Biocompatible Materials - chemical synthesis</topic><topic>Biocompatible Materials - chemistry</topic><topic>Biodegradable materials</topic><topic>Biodegradation, Environmental</topic><topic>Biomimetic Materials - chemical synthesis</topic><topic>Biomimetic Materials - chemistry</topic><topic>Blood Vessel Prosthesis</topic><topic>Cell Adhesion</topic><topic>Cell Adhesion Molecules - metabolism</topic><topic>Cell Culture Techniques</topic><topic>Cell Survival</topic><topic>Cells, Cultured</topic><topic>Collagen - metabolism</topic><topic>Coronary Vessels - cytology</topic><topic>Culture Media - chemistry</topic><topic>Culture Media - pharmacology</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - drug effects</topic><topic>Endothelium, Vascular - metabolism</topic><topic>Endothelium, Vascular - physiology</topic><topic>Endothelium, Vascular - ultrastructure</topic><topic>Gene Expression</topic><topic>Humans</topic><topic>Materials Testing</topic><topic>Nanotechnology</topic><topic>Polyesters - chemical synthesis</topic><topic>Polyesters - chemistry</topic><topic>Polymers</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Skin & tissue grafts</topic><topic>Spectrometry, X-Ray Emission</topic><topic>Spectroscopy, Fourier Transform Infrared</topic><topic>Tensile Strength</topic><topic>Time Factors</topic><topic>Tissue engineering</topic><topic>Tissue Engineering - methods</topic><toplevel>online_resources</toplevel><creatorcontrib>He, Wei</creatorcontrib><creatorcontrib>Yong, Thomas</creatorcontrib><creatorcontrib>Teo, Wee Eong</creatorcontrib><creatorcontrib>Ma, Zuwei</creatorcontrib><creatorcontrib>Ramakrishna, Seeram</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Tissue engineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>He, Wei</au><au>Yong, Thomas</au><au>Teo, Wee Eong</au><au>Ma, Zuwei</au><au>Ramakrishna, Seeram</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fabrication and Endothelialization of Collagen-Blended Biodegradable Polymer Nanofibers: Potential Vascular Graft for Blood Vessel Tissue Engineering</atitle><jtitle>Tissue engineering</jtitle><addtitle>Tissue Eng</addtitle><date>2005-09-01</date><risdate>2005</risdate><volume>11</volume><issue>9-10</issue><spage>1574</spage><epage>1588</epage><pages>1574-1588</pages><issn>1076-3279</issn><eissn>1557-8690</eissn><abstract>Electrospun collagen-blended poly(L-lactic acid)-
co
-poly(∈-caprolactone) [P(LLA-CL), 70:30]
nanofiber may have great potential application in tissue engineering because it mimicks the extracellular
matrix (ECM) both morphologically and chemically. Blended nanofibers with various weight
ratios of polymer to collagen were fabricated by electrospinning. The appearance of the blended
nanofibers was investigated by scanning electron microscopy and transmission electron microscopy.
The nanofibers exhibited a smooth surface and a narrow diameter distribution, with 60% of the
nanofibers having diameters between 100 and 200 nm. Attenuated total reflectance-Fourier transform
infrared spectra and X-ray photoelectron spectroscopy verified the existence of collagen molecules
on the surface of nanofibers. Human coronary artery endothelial cells (HCAECs) were seeded
onto the blended nanofibers for viability, morphogenesis, attachment, and phenotypic studies. Five
characteristic endothelial cell (EC) markers, including four types of cell adhesion molecule and one
EC-preferential gene (von Willebrand factor), were studied by reverse transcription-polymerase
chain reaction. Results showed that the collagen-blended polymer nanofibers could enhance the viability,
spreading, and attachment of HCAECs and, moreover, preserve the EC phenotype. The
blending electrospinning technique shows potential in refining the composition of polymer nanofibers
by adding various ingredients (e.g., growth factors) according to cell types to fabricate tissue-engineering
scaffold, particularly blood vessel-engineering scaffold.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>16259611</pmid><doi>10.1089/ten.2005.11.1574</doi><tpages>15</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Tissue engineering, 2005-09, Vol.11 (9-10), p.1574-1588 |
| issn | 1076-3279 1557-8690 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_17420914 |
| source | Mary Ann Liebert Online Subscription; MEDLINE |
| subjects | Biocompatible Materials - chemical synthesis Biocompatible Materials - chemistry Biodegradable materials Biodegradation, Environmental Biomimetic Materials - chemical synthesis Biomimetic Materials - chemistry Blood Vessel Prosthesis Cell Adhesion Cell Adhesion Molecules - metabolism Cell Culture Techniques Cell Survival Cells, Cultured Collagen - metabolism Coronary Vessels - cytology Culture Media - chemistry Culture Media - pharmacology Endothelium, Vascular - cytology Endothelium, Vascular - drug effects Endothelium, Vascular - metabolism Endothelium, Vascular - physiology Endothelium, Vascular - ultrastructure Gene Expression Humans Materials Testing Nanotechnology Polyesters - chemical synthesis Polyesters - chemistry Polymers Reverse Transcriptase Polymerase Chain Reaction Skin & tissue grafts Spectrometry, X-Ray Emission Spectroscopy, Fourier Transform Infrared Tensile Strength Time Factors Tissue engineering Tissue Engineering - methods |
| title | Fabrication and Endothelialization of Collagen-Blended Biodegradable Polymer Nanofibers: Potential Vascular Graft for Blood Vessel Tissue Engineering |
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