Rapid isolation of kestose by low-pressure chromatography after enzymatic synthesis with invertase

By using a two-step protocol involving flash chromatography on silica gel-60 and gel filtration on Biogel P2 we isolated 56 g pure kestose/L after enzymatic synthesis with 115 U invertase in media containing 50% (w/w) sucrose and 90% (v/v) butyl acetate. The isolated product is homogenous after high...

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Veröffentlicht in:	Biotechnology techniques 1999-09, Vol.13 (9), p.625-629
Hauptverfasser:	SOMIARI, R. I, BIELECKI, S
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology butyl acetate Fundamental and applied biological sciences. Psychology kestose Methods. Procedures. Technologies Others Various methods and equipments
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Zusammenfassung:	By using a two-step protocol involving flash chromatography on silica gel-60 and gel filtration on Biogel P2 we isolated 56 g pure kestose/L after enzymatic synthesis with 115 U invertase in media containing 50% (w/w) sucrose and 90% (v/v) butyl acetate. The isolated product is homogenous after high pH anion exchange chromatography with pulsed amperometric detection and is suitable for downstream utilization without further purification.
ISSN:	0951-208X 1573-6784
DOI:	10.1023/A:1008912230106