Identification and characterization of a type II restriction endonuclease, StrI from Streptomyces thermodiastaticus

Identification and characterization of a type II restriction endonuclease, StrI from Streptomyces thermodiastaticus

A new type II restriction endonuclease, StrI has been identified from Streptomyces thermodiastaticus. The enzyme has been purified using three column chromatography steps. The enzyme recognizes hexanucleotide sequence and cleaves DNA 5′-C↓TCGAG-3′ as indicated. The optimum temperature, pH, and catio...

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Veröffentlicht in:Current science (Bangalore) 1999-07, Vol.77 (2), p.273-276
Hauptverfasser: Chandrashekaran, Siddamadappa, Shankar, Aparna Bhavani, Babu, Padmanabhan, Paul, Bindu Diana, Nagaraja, Valakunja
Format: Artikel
Sprache:eng
Schlagworte:
Adenoviruses
Bacteria
deoxyribonuclease StrI
DNA
Enzyme activity
Enzymes
Nucleic acids
RESEARCH COMMUNICATIONS
Streptomyces
Streptomyces thermodiastaticus
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Zusammenfassung:A new type II restriction endonuclease, StrI has been identified from Streptomyces thermodiastaticus. The enzyme has been purified using three column chromatography steps. The enzyme recognizes hexanucleotide sequence and cleaves DNA 5′-C↓TCGAG-3′ as indicated. The optimum temperature, pH, and cation requirements for the enzyme activity were determined.
ISSN:0011-3891