Effect of thiobencarb on growth and photosynthesis of the soil alga Protosiphon botryoides (Chlorophyta)

The effects of the herbicide thiobencarb (Saturn) were tested on the growth and physiology of the chlorophyte Protosiphon botryoides isolated from an Egyptian paddy. Assays were conducted using 16-day batch cultures. Chlorophyll and dry weight biomass yields were significantly reduced at 2-3 mg L super(-1) thiobencarb, and dark respiration increased and protein decreased significantly at 3 mg L super(-1). Reductions in exponential specific growth rate ( mu ) were generally small, but in some cases significant. Thiobencarb also slightly, but significantly, reduced the 77 K fluorescence parameter F sub(v)/F sub(m), an indicator of maximum photosynthetic efficiency. No consistent dose-dependent changes occurred in chlorophyll per unit dry weight, total carbohydrate or gross photosynthetic capacity. Whereas half of the added thiobencarb was recovered from control (uninoculated) medium, it was largely absent from cells and culture medium after sixteen days, indicating biodegradation by the alga or associated bacteria. P. botryoides recovered fully within sixteen days following subculture in thiobencarb-free medium. Independently varying phosphate and nitrate nine-fold had no clear effec on the sensitivity of P. botryoides to thiobencarb.