Application of poly-β-hydroxybutyrate (PHB) in mussel larviculture

In this study, the effect of poly-β-hydroxybutyrate (PHB) delivery on the performance (survival, growth and metamorphosis) of blue mussel (Mytilus edulis) larvae was studied for the first time. Upon addition of PHB in either crystalline (i.e. extracted from the bacterial cell) or amorphous (i.e. sti...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Aquaculture 2015-09, Vol.446, p.318-324
Hauptverfasser: Hung, Nguyen Van, De Schryver, Peter, Tam, Tran Thanh, Garcia-Gonzalez, Linsey, Bossier, Peter, Nevejan, Nancy
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:In this study, the effect of poly-β-hydroxybutyrate (PHB) delivery on the performance (survival, growth and metamorphosis) of blue mussel (Mytilus edulis) larvae was studied for the first time. Upon addition of PHB in either crystalline (i.e. extracted from the bacterial cell) or amorphous (i.e. still contained in the bacterial cell) forms to a standard algal diet at concentrations of 0.1mgL−1, 1.0mgL−1 and 10.0mgL−1, no significant improvement in growth performance or metamorphosis was observed. However, a concentration of 1.0mgL−1 amorphous PHB almost doubled the survival of the larvae in several independent experiments. A total of sixteen PHB-degrading bacterial strains belonging to genus Pseudoalteromonas could be isolated from the PHB treated mussel larvae. The application of these in combination with PHB however, did not further increase the culture performance of the mussel larvae. Where previous studies suggested that the PHB effect could be related to a change in the intestinal microbial community of the treated animals, molecular fingerprinting analysis of the mussel larvae associated microbiota did not show a relationship between changes in the microbiota composition and the improved survival following PHB treatment. Overall, this study showed for the first time that PHB can be applied in blue mussel larviculture to increase the survival of the cultured animals. Novel approach to increase survival in mussel larviculture. •First study on the application of PHB in mussel larviculture•PHB had a positive effect on the survival but not on the growth of blue mussel (Mytilus edulis) larvae and spat.•Amorphous PHB performs better than crystalline PHB in mussel larviculture.•Gut microbiota in mussel larvae was not significantly modulated by PHB supplementation and therefore could not be related to larval performance.•The use of PHB-degrading bacteria provides no added value; it even surprisingly suppressed larval growth.
ISSN:0044-8486
1873-5622
DOI:10.1016/j.aquaculture.2015.04.036