Inorganic mercury pre-exposures protect against methyl mercury toxicity in NSC-34 (neuron×spinal cord hybrid) cells
A neuron spinal chord × hybrid (NSC-34) cell culture derived from neonatal mouse was characterized for studies on mercury toxicity. Exposure of NSC-34 cells to methyl mercury chloride (MeHgCl) (0–16 μM) resulted in significant dose-dependent cell damage and death ( P
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| Veröffentlicht in: | Toxicology (Amsterdam) 1999-02, Vol.132 (2), p.167-178 |
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| creator | Chapman, Laurie Ann Chan, Hing Man |
| description | A neuron spinal chord × hybrid (NSC-34) cell culture derived from neonatal mouse was characterized for studies on mercury toxicity. Exposure of NSC-34 cells to methyl mercury chloride (MeHgCl) (0–16 μM) resulted in significant dose-dependent cell damage and death (
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| doi_str_mv | 10.1016/S0300-483X(98)00151-6 |
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P<0.05). MeHgCl was more toxic than inorganic mercury (Hg
2+) for both the NSC-34 cells and its parent neuroblastoma cell line N18TG-2 (
P<0.05). Hg
2+, but not ZnCl
2 or MeHg exposure induced metallothionein (MT) (
P<0.05). To mimic the increase in Hg
2+in the mammalian brain with long term MeHg exposure, the cells were treated with 1 μM mercuric chloride (HgCl
2) for five passages before exposure to MeHgCl (1–16 μM) for 48 h. MeHgCl toxicity was measured by trypan blue exclusion, reduction of resazurin dye and acid phosphatase activity. Pre-exposure to HgCl
2 lessened the toxicity as shown by trypan blue exclusion (
P=0.0559) and reduction of resazurin (
P=0.0001). Pre-exposure to HgCl
2 also resulted in induction of MT (
P=0.0066) and lessened the decrease of reduced glutathione (GSH) (
P=0.0013). These results suggest that MT and GSH may play a protective role in methyl mercury induced neurotoxicity of neuron spinal chord cells. The NSC-34 hybrid cell line can be a useful model for the study of MeHg neurotoxicity.</description><identifier>ISSN: 0300-483X</identifier><identifier>EISSN: 1879-3185</identifier><identifier>DOI: 10.1016/S0300-483X(98)00151-6</identifier><identifier>PMID: 10433380</identifier><identifier>CODEN: TXICDD</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Acid Phosphatase - metabolism ; Animals ; Biological and medical sciences ; Cells, Cultured ; Chemical and industrial products toxicology. Toxic occupational diseases ; Cultured neurons ; glutathione ; Glutathione - analysis ; Hybrid Cells ; Inorganic mercury ; Medical sciences ; mercuric chloride ; Mercury - pharmacology ; Metallothionein ; Metallothionein - analysis ; Metals and various inorganic compounds ; Methyl mercury ; Methylmercury Compounds - toxicity ; Mice ; Neurons - drug effects ; Neurotoxicity ; Spinal Cord - drug effects ; Toxicology ; Zinc ; Zinc - pharmacology</subject><ispartof>Toxicology (Amsterdam), 1999-02, Vol.132 (2), p.167-178</ispartof><rights>1999 Elsevier Science Ireland Ltd</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-b898720a2703ef1c0036efec5a41f30f61293c51d1bad284614fea9211c433b93</citedby><cites>FETCH-LOGICAL-c504t-b898720a2703ef1c0036efec5a41f30f61293c51d1bad284614fea9211c433b93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0300483X98001516$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1741679$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10433380$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chapman, Laurie Ann</creatorcontrib><creatorcontrib>Chan, Hing Man</creatorcontrib><title>Inorganic mercury pre-exposures protect against methyl mercury toxicity in NSC-34 (neuron×spinal cord hybrid) cells</title><title>Toxicology (Amsterdam)</title><addtitle>Toxicology</addtitle><description>A neuron spinal chord × hybrid (NSC-34) cell culture derived from neonatal mouse was characterized for studies on mercury toxicity. Exposure of NSC-34 cells to methyl mercury chloride (MeHgCl) (0–16 μM) resulted in significant dose-dependent cell damage and death (
P<0.05). MeHgCl was more toxic than inorganic mercury (Hg
2+) for both the NSC-34 cells and its parent neuroblastoma cell line N18TG-2 (
P<0.05). Hg
2+, but not ZnCl
2 or MeHg exposure induced metallothionein (MT) (
P<0.05). To mimic the increase in Hg
2+in the mammalian brain with long term MeHg exposure, the cells were treated with 1 μM mercuric chloride (HgCl
2) for five passages before exposure to MeHgCl (1–16 μM) for 48 h. MeHgCl toxicity was measured by trypan blue exclusion, reduction of resazurin dye and acid phosphatase activity. Pre-exposure to HgCl
2 lessened the toxicity as shown by trypan blue exclusion (
P=0.0559) and reduction of resazurin (
P=0.0001). Pre-exposure to HgCl
2 also resulted in induction of MT (
P=0.0066) and lessened the decrease of reduced glutathione (GSH) (
P=0.0013). These results suggest that MT and GSH may play a protective role in methyl mercury induced neurotoxicity of neuron spinal chord cells. The NSC-34 hybrid cell line can be a useful model for the study of MeHg neurotoxicity.</description><subject>Acid Phosphatase - metabolism</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Chemical and industrial products toxicology. Toxic occupational diseases</subject><subject>Cultured neurons</subject><subject>glutathione</subject><subject>Glutathione - analysis</subject><subject>Hybrid Cells</subject><subject>Inorganic mercury</subject><subject>Medical sciences</subject><subject>mercuric chloride</subject><subject>Mercury - pharmacology</subject><subject>Metallothionein</subject><subject>Metallothionein - analysis</subject><subject>Metals and various inorganic compounds</subject><subject>Methyl mercury</subject><subject>Methylmercury Compounds - toxicity</subject><subject>Mice</subject><subject>Neurons - drug effects</subject><subject>Neurotoxicity</subject><subject>Spinal Cord - drug effects</subject><subject>Toxicology</subject><subject>Zinc</subject><subject>Zinc - pharmacology</subject><issn>0300-483X</issn><issn>1879-3185</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctu1DAUhi0EokPhEUBeoKpdBM6JndsKoRG9SBUsChI7y3FOWqOMPdhO1TwJD8SLNemMCruuLEvfsc__f4y9RfiAgOXHKxAAmazFz-OmPgHAArPyGVthXTWZwLp4zlaPyAF7FeMvAMiFLF-yAwQphKhhxdKF8-FaO2v4hoIZw8S3gTK62_o4BorzzScyietrbV1MM5VupuERTv7OGpsmbh3_erXOhOTHjsbg3d8_cWudHrjxoeM3Uxtsd8INDUN8zV70eoj0Zn8esh-nX76vz7PLb2cX68-XmSlApqytm7rKQecVCOrRAIiSejKFltgL6EvMG2EK7LDVXV7LEmVPuskRzRyvbcQhO9q9O4f4PVJMamPjsoF25MeosBLF3EP5NCgLaKp8AYsdaIKPMVCvtsFudJgUglq8qAcvaildNbV68KKWuXf7D8Z2Q91_UzsRM_B-D-ho9NAH7YyN_7hKYlktiT7tMJpru7UUVDSWnKHOhtmS6rx9YpN770GrPQ</recordid><startdate>19990215</startdate><enddate>19990215</enddate><creator>Chapman, Laurie Ann</creator><creator>Chan, Hing Man</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>C1K</scope><scope>SOI</scope><scope>7TK</scope><scope>7U7</scope></search><sort><creationdate>19990215</creationdate><title>Inorganic mercury pre-exposures protect against methyl mercury toxicity in NSC-34 (neuron×spinal cord hybrid) cells</title><author>Chapman, Laurie Ann ; Chan, Hing Man</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c504t-b898720a2703ef1c0036efec5a41f30f61293c51d1bad284614fea9211c433b93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Acid Phosphatase - metabolism</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Chemical and industrial products toxicology. Toxic occupational diseases</topic><topic>Cultured neurons</topic><topic>glutathione</topic><topic>Glutathione - analysis</topic><topic>Hybrid Cells</topic><topic>Inorganic mercury</topic><topic>Medical sciences</topic><topic>mercuric chloride</topic><topic>Mercury - pharmacology</topic><topic>Metallothionein</topic><topic>Metallothionein - analysis</topic><topic>Metals and various inorganic compounds</topic><topic>Methyl mercury</topic><topic>Methylmercury Compounds - toxicity</topic><topic>Mice</topic><topic>Neurons - drug effects</topic><topic>Neurotoxicity</topic><topic>Spinal Cord - drug effects</topic><topic>Toxicology</topic><topic>Zinc</topic><topic>Zinc - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chapman, Laurie Ann</creatorcontrib><creatorcontrib>Chan, Hing Man</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><jtitle>Toxicology (Amsterdam)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chapman, Laurie Ann</au><au>Chan, Hing Man</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inorganic mercury pre-exposures protect against methyl mercury toxicity in NSC-34 (neuron×spinal cord hybrid) cells</atitle><jtitle>Toxicology (Amsterdam)</jtitle><addtitle>Toxicology</addtitle><date>1999-02-15</date><risdate>1999</risdate><volume>132</volume><issue>2</issue><spage>167</spage><epage>178</epage><pages>167-178</pages><issn>0300-483X</issn><eissn>1879-3185</eissn><coden>TXICDD</coden><abstract>A neuron spinal chord × hybrid (NSC-34) cell culture derived from neonatal mouse was characterized for studies on mercury toxicity. Exposure of NSC-34 cells to methyl mercury chloride (MeHgCl) (0–16 μM) resulted in significant dose-dependent cell damage and death (
P<0.05). MeHgCl was more toxic than inorganic mercury (Hg
2+) for both the NSC-34 cells and its parent neuroblastoma cell line N18TG-2 (
P<0.05). Hg
2+, but not ZnCl
2 or MeHg exposure induced metallothionein (MT) (
P<0.05). To mimic the increase in Hg
2+in the mammalian brain with long term MeHg exposure, the cells were treated with 1 μM mercuric chloride (HgCl
2) for five passages before exposure to MeHgCl (1–16 μM) for 48 h. MeHgCl toxicity was measured by trypan blue exclusion, reduction of resazurin dye and acid phosphatase activity. Pre-exposure to HgCl
2 lessened the toxicity as shown by trypan blue exclusion (
P=0.0559) and reduction of resazurin (
P=0.0001). Pre-exposure to HgCl
2 also resulted in induction of MT (
P=0.0066) and lessened the decrease of reduced glutathione (GSH) (
P=0.0013). These results suggest that MT and GSH may play a protective role in methyl mercury induced neurotoxicity of neuron spinal chord cells. The NSC-34 hybrid cell line can be a useful model for the study of MeHg neurotoxicity.</abstract><cop>Shannon</cop><cop>Amsterdam</cop><pub>Elsevier Ireland Ltd</pub><pmid>10433380</pmid><doi>10.1016/S0300-483X(98)00151-6</doi><tpages>12</tpages></addata></record> |
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| subjects | Acid Phosphatase - metabolism Animals Biological and medical sciences Cells, Cultured Chemical and industrial products toxicology. Toxic occupational diseases Cultured neurons glutathione Glutathione - analysis Hybrid Cells Inorganic mercury Medical sciences mercuric chloride Mercury - pharmacology Metallothionein Metallothionein - analysis Metals and various inorganic compounds Methyl mercury Methylmercury Compounds - toxicity Mice Neurons - drug effects Neurotoxicity Spinal Cord - drug effects Toxicology Zinc Zinc - pharmacology |
| title | Inorganic mercury pre-exposures protect against methyl mercury toxicity in NSC-34 (neuron×spinal cord hybrid) cells |
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