Development of an improved ESAT-6 and CFP-10 peptide-based cytokine flow cytometric assay for bovine tuberculosis

Highlights • We developed an improved cytokine flow cytometric assay for bovine tuberculosis. • IFN-γ detected in whole blood cultures in infected cattle 6 h post stimulation. • Using ESAT-6/CFP10 in the assay improved both sensitivity and specificity. • Responding cells were CD45R0+CD69+CD4+ memory...

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Veröffentlicht in:	Comparative immunology, microbiology and infectious diseases microbiology and infectious diseases, 2015-10, Vol.42, p.1-7
Hauptverfasser:	Elnaggar, Mahmoud M, Abdellrazeq, Gaber S, Sester, Martina, Khaliel, Samy A, Singh, Mahavir, Torky, Helmy A, Davis, William C
Format:	Artikel
Sprache:	eng
Schlagworte:	Allergy and Immunology Animals Antigens, Bacterial - immunology Antigens, CD - immunology Antigens, Differentiation, T-Lymphocyte - immunology Bacterial Proteins - immunology Bovine tuberculosis Cattle CD4-Positive T-Lymphocytes - immunology Cytokine flow cytometry ESAT-6/CFP-10 Flow Cytometry - methods IFN-γ release assay Immunologic Memory Infectious Disease Interferon-gamma - blood Interferon-gamma Release Tests - methods Lectins, C-Type - immunology Leukocyte Common Antigens - immunology Lymphokines Mycobacterium bovis Mycobacterium bovis - immunology Peptides - immunology Sensitivity and Specificity Tuberculin Test Tuberculosis, Bovine - diagnosis
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Zusammenfassung:	Highlights • We developed an improved cytokine flow cytometric assay for bovine tuberculosis. • IFN-γ detected in whole blood cultures in infected cattle 6 h post stimulation. • Using ESAT-6/CFP10 in the assay improved both sensitivity and specificity. • Responding cells were CD45R0+CD69+CD4+ memory T cells. • The assay provides a new opportunity to track the immune response.
ISSN:	0147-9571 1878-1667
DOI:	10.1016/j.cimid.2015.07.005