Quantitative trait loci associated with kernel traits in a soft x hard wheat cross

Kernel morphology and texture influence the value of wheat (Triticum aestivum L.). The objectives of this study were to determine associations between kernel traits and molecular markers and to identify quantitative trait loci (QTLs) affecting kernel traits in a soft x hard white wheat cross. Seventy eight F(2.5)-derived recombinant inbred lines (RILs) from a cross between the soft white wheat NY6432-18 (NY18) and the hard white wheat 'Clark's Cream' (CC) were developed by single seed descent. Kernel texture was measured by near infrared reflectance (NIR) on RIL grain samples from six environments. Digital image analysis (DIA) was used to measure kernel length, width, area, perimeter on grain samples from four environments. Test weight and thousand kernel weight (TKW) were also determined. Shape factor and density factor were calculated. The map for this population consisted of 313 molecular markers in 47 linkage groups located on all wheat homoeologous chromosome groups. Linkage groups that mapped to wheat homoeologous group 2 chromosomes were highly skewed towards NY18 alleles. Genotype effects and genotype x environment interactions were highly significant for most traits. QTLs for kernel width and kernel length also influenced kernel area and TKW, but did not influence each other. The pinB marker at the puroindoline B locus on chromosome 5DS explained over 60% of the phenotypic variation for kernel texture. QTLs for kernel traits were located on chromosomes 1A, 2B, 2D, 3B, 7A, and 7B.