Red-shifted red/green-type cyanobacteriochrome AM1_1870g3 from the chlorophyll d-bearing cyanobacterium Acaryochloris marina

Cyanobacteriochromes (CBCRs) are diverse photoreceptors that are found only from cyanobacteria and cover wide range of light qualities. CBCRs are divided into two types regarding the chromophore species they contain: phycocyanobilin (PCB) and phycoviolobilin. Red/green-type CBCRs are widely distributed subfamily among the PCB-binding CBCRs and photoconvert between a red-absorbing thermostable form and a green-absorbing metastable form. Our recent study discovered that a red/green-type CBCR, AM1_1557g2, from a cyanobacterium Acaryochloris marina covalently binds not only PCB but also biliverdin (BV). BV-binding AM1_1557g2 photoconverts between a far-red absorbing form and an orange-absorbing form. We report, herein, that another red/green-type CBCR, AM1_1870g3, from the cyanobacterium A. marina also bound both PCB and BV. PCB- and BV-binding ones showed red/green and far-red/orange reversible photoconversions, respectively. Unexpectedly, absorbing wavelengths are 10–20 nm red-shifted compared with those of AM1_1557g2. These red-shifted characteristics may be useful for optogenetic light switches that work in various organisms. •Cyanobacteriochrome AM1_1870g3 binds not only phycocyanobilin but also biliverdin.•Phycocyanobilin-binding AM1_1870g3 shows red/green reversible photoconversion.•Biliverdin-binding AM1_1870g3 shows far-red/orange reversible photoconversion.•Both phycocyanobilin- and biliverdin-binding AM1_1870g3 possess red-shifted feature.