Regulatory T-cell subsets in response to specific Mycobacterium tuberculosis antigens in vitro distinguish among individuals with different QTF and TST reactivity

Abstract Regulatory T cells (Tregs), a subset of CD4 + T cells related with immune regulation, have been associated with active and latent tuberculosis infection (LTBI). Treg frequencies were evaluated by multicolor flow cytometry (FC) in peripheral blood mononuclear cells (PBMCs) stimulated with my...

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Veröffentlicht in:	Clinical immunology (Orlando, Fla.) Fla.), 2015-04, Vol.157 (2), p.145-155
Hauptverfasser:	Serrano, C.J, Castañeda-Delgado, J.E, Trujillo-Ochoa, J.L, González-Amaro, R, García-Hernández, M.H, Enciso-Moreno, J.A
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Allergy and Immunology Antigens, Bacterial - immunology Antigens, CD - metabolism Apyrase - metabolism CD4-Positive T-Lymphocytes - immunology CD4-Positive T-Lymphocytes - metabolism Cohort Studies Female Forkhead Transcription Factors - metabolism Humans In Vitro Techniques Interferon-gamma Release Tests Interleukin-2 Receptor alpha Subunit - metabolism Interleukin-7 Receptor alpha Subunit - metabolism Latent Tuberculosis - immunology Leukocytes, Mononuclear Male Middle Aged Mycobacterium tuberculosis Mycobacterium tuberculosis - immunology QFT Regulatory T cells T-Lymphocyte Subsets - immunology T-Lymphocyte Subsets - metabolism T-Lymphocytes, Regulatory - immunology T-Lymphocytes, Regulatory - metabolism TST Tuberculin Test Tuberculosis Tuberculosis, Pulmonary - transmission Young Adult
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Zusammenfassung:	Abstract Regulatory T cells (Tregs), a subset of CD4 + T cells related with immune regulation, have been associated with active and latent tuberculosis infection (LTBI). Treg frequencies were evaluated by multicolor flow cytometry (FC) in peripheral blood mononuclear cells (PBMCs) stimulated with mycobacterial antigens ESAT-6, CFP-10, and TB7.7 to assess their capacity to distinguish subjects with different reactivity to the QuantiFERON-TB® Gold In-Tube (QFT-IT) test and the tuberculin skin test (TST). Increased frequencies of CD4 + CD25highCD39 + cells were found for the [TST +, QTF +] compared with the [TST +, QTF −] group. Also, higher frequencies were observed for the [TST +, QTF +] compared with the [TST +, QTF −] and [TST −, QTF −] groups in CD4 + CD25highFoxp3 + and CD4 + CD25highCD39 + Foxp3 + populations. Receiver operating characteristics (ROC curve) analysis confirmed these discriminating results. QFT–IT and TST quantitative values correlated with several Treg population frequencies.
ISSN:	1521-6616 1521-7035
DOI:	10.1016/j.clim.2015.02.008