Mouse Dendritic Cells Express the P2X sub(7) Purinergic Receptor: Characterization and Possible Participation in Antigen Presentation

Immune cells express P2 purinoceptors of the P2Y and P2X subtypes. In the present work, we show that three dendritic cell (DC) lines, D2SC/1, CB1, and FSDC, representative of immature DCs, express the P2X sub(7) (formerly P2Z) receptor, as judged from RT-PCR amplification, reactivity to a specific a...

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Veröffentlicht in:The Journal of immunology (1950) 1999-08, Vol.163 (4), p.1958-1965
Hauptverfasser: Mutini, C, Falzoni, S, Ferrari, D, Chiozzi, P, Morelli, A, Baricordi, O R, Collo, G, Ricciardi-Castagnoli, P, Di Virgilio, F
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Sprache:eng
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Zusammenfassung:Immune cells express P2 purinoceptors of the P2Y and P2X subtypes. In the present work, we show that three dendritic cell (DC) lines, D2SC/1, CB1, and FSDC, representative of immature DCs, express the P2X sub(7) (formerly P2Z) receptor, as judged from RT-PCR amplification, reactivity to a specific antiserum, and pharmacological and functional evidence. Receptor expression is higher in FSDC cells, a cell line that is functionally more mature than D2SC/1 and CB1. From the wild-type DC population, we selected cell clones lacking the P2X sub(7)R (P2X sub(7)less). We also used a P2XR blocker, oxidized ATP, to irreversibly inhibit the P2X sub(7)R. Ability of P2X sub(7)less FSDCs or of oxidized ATP-inhibited FSDCs to stimulate Ag-specific T sub(H) lymphocytes was severely decreased although Ag endocytosis was minimally affected. During coculture with T sub(H) lymphocytes, wild-type FSDC secreted large amounts of IL-1 beta . Release of this cytokine was reduced in P2X sub(7)less DCs. These data show that DCs express the P2X sub(7) purinoceptor and suggest a correlation between P2X sub(7)R expression and Ag-presenting activity.
ISSN:0022-1767