Granulosa cell proliferation in the gilt ovary associated with ovarian status and porcine reproductive and respiratory syndrome virus detection

The aims of the present study were to quantify the number of follicles and determine granulosa cell proliferation in the ovaries of gilts in relation to ovarian status and porcine reproductive and respiratory syndrome (PRRS) virus infection. Ovarian tissues were obtained from 37 Landrace × Yorkshire crossbred gilts aged 270.7 ± 24.5 days and weighing 144.5 ± 15.0 kg (27 cycling and 10 prepubertal gilts). PRRS virus was detected using immunohistochemistry, and the ovaries were classified as negative ( n = 17) or positive ( n = 20) to PRRS virus. Granulosa cell proliferation was determined by proliferating cell nuclear antigen (PCNA) immunostaining. The proportion of PCNA-expressing granulosa cells in pre-antral ( n = 197) and antral follicles ( n = 76) was determined using Image-Pro® Plus software. The number of follicles in ovarian tissue containing PRRS virus did not differ significantly compared with those without PRRS virus. Granulosa cell proliferation was influenced by the type of follicle ( P