In vitro incubation of human spermatozoa promotes reactive oxygen species generation and DNA fragmentation

In vitro incubation of human spermatozoa promotes reactive oxygen species generation and DNA fragmentation

Summary The aim of this study was to investigate the oxidative process associated with sperm capacitation and its impact on DNA fragmentation and sperm function. Redox activity and lipid peroxidation were analysed in human spermatozoa after 3, 6 and 22 h of incubation in Ham′s F10 medium plus bovine...

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Veröffentlicht in:Andrologia 2015-10, Vol.47 (8), p.861-866
Hauptverfasser: Cicaré, J., Caille, A., Zumoffen, C., Ghersevich, S., Bahamondes, L., Munuce, M. J.
Format: Artikel
Sprache:eng
Schlagworte:
Acrosome Reaction
DNA Fragmentation
dynamics
Humans
In Vitro Techniques
Lipid Peroxidation
Male
Oxidation-Reduction
Reactive Oxygen Species - analysis
Reproductive Techniques, Assisted - adverse effects
SCD test
sperm
Sperm Capacitation
Spermatozoa - chemistry
Spermatozoa - metabolism
Spermatozoa - physiology
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Zusammenfassung:Summary The aim of this study was to investigate the oxidative process associated with sperm capacitation and its impact on DNA fragmentation and sperm function. Redox activity and lipid peroxidation were analysed in human spermatozoa after 3, 6 and 22 h of incubation in Ham′s F10 medium plus bovine albumin at 37° and 5% CO2 for capacitation. DNA status, tyrosine phosphorylation pattern and induced acrosome reaction were evaluated after capacitating conditions. At 22 h of incubation, there was a significant (P 
ISSN:0303-4569
1439-0272
DOI:10.1111/and.12337