Easter recombinant DNA procedures for Streptomyces

The great industrial importance of the streptomycetes has stimulated their genetic manipulation. The plasmid or phage vectors that have been developed require recombinant selection and screening procedures, which in Streptomyces can take several days and necessitate comparatively large volumes of cultures and reagents. To improve the situation, we have coupled a scaled-down procedure for DNA isolation with polymerase chain reaction amplification.