Extraction of DNA from human skeletal remains: practical applications in forensic sciences

Extraction of DNA from human skeletal remains: practical applications in forensic sciences

We have developed an efficient protocol for the extraction of DNA from bone samples. The protocol precludes decalcification and associates an efficient extraction of the DNA with removal of inhibitors by binding of DNA to silica particles. Two forensic cases are described in detail to demonstrate th...

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Veröffentlicht in:Genetic analysis : biomolecular engineering 1997-07, Vol.14 (2), p.41-44
Hauptverfasser: Prado, V.F, Castro, A.K.F, Oliveira, C.L, Souza, K.T, Pena, S.D.J
Format: Artikel
Sprache:eng
Schlagworte:
Bone
Bone and Bones - chemistry
Bone and Bones - pathology
Child
DNA extraction
DNA typing
DNA, Mitochondrial - analysis
Female
Forensic Medicine
Homicide
Human identification
Humans
Male
Microsatellites
Mitochondrial DNA
Paternity
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Beschreibung
Zusammenfassung:We have developed an efficient protocol for the extraction of DNA from bone samples. The protocol precludes decalcification and associates an efficient extraction of the DNA with removal of inhibitors by binding of DNA to silica particles. Two forensic cases are described in detail to demonstrate the usefulness of the method.
ISSN:1050-3862
DOI:10.1016/S1050-3862(97)00004-1