Purification and characterisation of PQQ-dependent glucose dehydrogenase from Erwinia sp. 34-1

Purification and characterisation of PQQ-dependent glucose dehydrogenase from Erwinia sp. 34-1

A pyrroloquinoline quinone-dependent glucose dehydrogenase from an isolate of Erwinia sp. has been purified to homogeneity and characterised. SDS-PAGE showed a single band of 88.4 kDa. The enzyme activity was optimal at 47°C and pH 7.5-8.5. The Michaelis constants for d-glucose and PMS were 3.2 mM a...

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Veröffentlicht in:Biotechnology letters 1999-03, Vol.21 (3), p.187-192
Hauptverfasser: MARCINKEVICIENE, L, BACHMATOVA, I, SEMENAITE, R, RUDOMANSKIS, R, BRAZENAS, G, MESKIENE, R, MESKYS, R
Format: Artikel
Sprache:eng
Schlagworte:
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Dehydrogenase
Enzymatic activity
Erwinia
Fundamental and applied biological sciences. Psychology
Microbiology
Miscellaneous
Mission oriented research
Sodium hydroxide
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Zusammenfassung:A pyrroloquinoline quinone-dependent glucose dehydrogenase from an isolate of Erwinia sp. has been purified to homogeneity and characterised. SDS-PAGE showed a single band of 88.4 kDa. The enzyme activity was optimal at 47°C and pH 7.5-8.5. The Michaelis constants for d-glucose and PMS were 3.2 mM and 132 μM, respectively (50 mM glycine-NaOH, at pH 8.0).[PUBLICATION ABSTRACT]
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1005499709935