Cloning and nucleotide sequence of one of the most highly expressed genes, a pdcA homolog of Aspergillus nidulans, in Aspergillus oryzae

A homolog of Aspergillus nidulans pdcA that is probably one of the most highly expressed in Aspergillus oryzae ATCC 22788 was isolated, as measured by the frequency among randomly selected 324 expressed sequence tags. It has an 1,632 bp open reading frame for a polypeptide of about 60 kDa. Its amino...

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Veröffentlicht in:	Biotechnology letters 1999-02, Vol.21 (2), p.139-142
Hauptverfasser:	Lee, D.W, Koh, J.S, Kim, J.H, Chae, K.S
Format:	Artikel
Sprache:	eng
Schlagworte:	amino acid sequences Amino acids Aspergillus nidulans Aspergillus oryzae Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology Cloning comparisons Enzymes Fundamental and applied biological sciences. Psychology genbank/af098293 gene expression Genetics Mission oriented research molecular sequence data nucleotide sequences Proteins
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Zusammenfassung:	A homolog of Aspergillus nidulans pdcA that is probably one of the most highly expressed in Aspergillus oryzae ATCC 22788 was isolated, as measured by the frequency among randomly selected 324 expressed sequence tags. It has an 1,632 bp open reading frame for a polypeptide of about 60 kDa. Its amino acid sequence revealed 74% identity and 84% similarity to that of A. nidulans pyruvate decarboxylase.
ISSN:	0141-5492 1573-6776
DOI:	10.1023/A:1005438505623