Preclinical Comparison of the Amyloid-β Radioligands [11C]Pittsburgh compound B and [18F]florbetaben in Aged APPPS1-21 and BRI1-42 Mouse Models of Cerebral Amyloidosis
Purpose The aim of this study was to compare [ 11 C]Pittsburgh compound B ([ 11 C]PiB) and [ 18 F]florbetaben ([ 18 F]FBB) for preclinical investigations of amyloid-β pathology. Procedures We investigated two aged animal models of cerebral amyloidosis with contrasting levels of amyloid-β relating to...
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Veröffentlicht in: | Molecular imaging and biology 2015-10, Vol.17 (5), p.688-696 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Purpose
The aim of this study was to compare [
11
C]Pittsburgh compound B ([
11
C]PiB) and [
18
F]florbetaben ([
18
F]FBB) for preclinical investigations of amyloid-β pathology.
Procedures
We investigated two aged animal models of cerebral amyloidosis with contrasting levels of amyloid-β relating to “high” (APPPS1-21
n
= 6, wild type (WT)
n
= 7) and “low” (BRI1-42
n
= 6, WT
n
= 6) target states, respectively.
Results
APPPS1-21 mice (
high target state
) demonstrated extensive fibrillar amyloid-β deposition that translated to significantly increased retention of [
11
C]PiB and [
18
F]FBB in comparison to their wild type. The retention pattern of [
11
C]PiB and [
18
F]FBB in this cohort displayed a significant correlation. However, the relative difference in tracer uptake between diseased and healthy mice was substantially higher for [
11
C]PiB than for [
18
F]FBB. Although immunohistochemistry confirmed the high plaque load in APPPS1-21 mice, correlation between tracer uptake and
ex vivo
quantification of amyloid-β was poor for both tracers. BRI1-42 mice (
low target state
) did not demonstrate increased tracer uptake.
Conclusions
In cases of high fibrillar amyloid-β burden, both tracers detected significant differences between diseased and healthy mice, with [
11
C]PiB showing a larger dynamic range. |
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ISSN: | 1536-1632 1860-2002 |
DOI: | 10.1007/s11307-015-0833-9 |