Sequence Analysis of lip R: A Good Method for Molecular Epidemiology of Clinical Isolates of Mycobacterium tuberculosis

Advances in DNA sequencing have greatly enhanced the molecular epidemiology studies. In order to assess evolutionary and phylogenetic relation of Mycobacterium tuberculosis isolates several gene targets were evaluated. In this study, appropriate fragments of 5 highly variable genes (rpsL, mprA, lipR...

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Veröffentlicht in:	Current microbiology 2015-10, Vol.71 (4), p.443-448
Hauptverfasser:	Saedi, Samaneh, Youssefi, Masoud, Safdari, Hadi, Soleimanpour, Saman, Marouzi, Parviz, Ghazvini, Kiarash
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - genetics Biomedical and Life Sciences Biotechnology Epidemiology Genetic Variation Genotype Life Sciences Microbiology Molecular Epidemiology - methods Molecular Typing - methods Mycobacterium tuberculosis Mycobacterium tuberculosis - classification Mycobacterium tuberculosis - genetics Phylogenetics Phylogeny Sequence Analysis, DNA - methods Sequence Homology Tuberculosis
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Beschreibung
Zusammenfassung:	Advances in DNA sequencing have greatly enhanced the molecular epidemiology studies. In order to assess evolutionary and phylogenetic relation of Mycobacterium tuberculosis isolates several gene targets were evaluated. In this study, appropriate fragments of 5 highly variable genes (rpsL, mprA, lipR, katG, and fgd1 genes) were sequenced. The sequence data were analyzed with neighbor-joining method using mega and Geneious software. The phylogenetic trees analyzes revealed that the discriminatory power of lipR is much stronger than that observed in the other genes. lipR could distinguish between more clinical isolates. Therefore, lipR is a promising target for sequence analyzes of M. tuberculosis.
ISSN:	0343-8651 1432-0991
DOI:	10.1007/s00284-015-0856-0