Antioxidant effects of clove bud (Syzygium aromaticum) extract used with different extenders on ram spermatozoa during cryopreservation

Antioxidant effects of clove bud (Syzygium aromaticum) extract used with different extenders on ram spermatozoa during cryopreservation

Clove bud (Syzygium aromaticum) extract was added at concentrations of 0, 35, 75, and 115μg/ml to ovine semen extenders in order to investigate the antioxidant activities of clove bud extract and its effects on semen quality parameters after cryopreservation of ram spermatozoa. The basic extender wa...

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Veröffentlicht in:Cryobiology 2014-12, Vol.69 (3), p.482-487
Hauptverfasser: Baghshahi, H., Riasi, A., Mahdavi, A.H., Shirazi, A.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Antioxidant
Antioxidants - chemistry
Antioxidants - metabolism
Biphenyl Compounds - metabolism
Clove bud
Cryopreservation
Cryopreservation - methods
Cryopreservation - veterinary
Cryoprotective Agents - chemistry
Cryoprotective Agents - metabolism
Detergent
Detergents - metabolism
Egg Yolk - metabolism
Eugenia
LDL
Lipoproteins, LDL - metabolism
Male
Picrates - metabolism
Plant Extracts - chemistry
Plant Extracts - metabolism
Ram
Semen
Semen Analysis
Semen Preservation - methods
Semen Preservation - veterinary
Sheep - physiology
Sodium Dodecyl Sulfate - metabolism
Sperm Motility - drug effects
Spermatozoa - cytology
Spermatozoa - drug effects
Spermatozoa - metabolism
Syzygium - chemistry
Syzygium aromaticum
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Zusammenfassung:Clove bud (Syzygium aromaticum) extract was added at concentrations of 0, 35, 75, and 115μg/ml to ovine semen extenders in order to investigate the antioxidant activities of clove bud extract and its effects on semen quality parameters after cryopreservation of ram spermatozoa. The basic extender was composed of Tris, egg yolk, and glycerol. Two other extenders were prepared by substitution of egg yolk with either LDL or egg yolk+SDS. The DPPH inhibition test was employed to assess the antioxidant activity of clove bud extract. Results showed that, compared to vitamin E, clove bud extract had a higher antioxidant activity. Better sperm motility and movement characteristics (P
ISSN:0011-2240
1090-2392
DOI:10.1016/j.cryobiol.2014.10.009