High-throughput functional screening reveals low frequency of antibiotic resistance genes in DNA recovered from the Upper Mississippi River

In this study, we determined the frequency of antibiotic resistance genes (ARGs) in the Upper Mississippi River using a high-throughput, functional, metagenomic screening procedure. Fosmid libraries containing ∼10,000 clones were screened for resistance to ampicillin, cephalothin, kanamycin, and tet...

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Veröffentlicht in:	Journal of water and health 2015-09, Vol.13 (3), p.693-703
Hauptverfasser:	Staley, Christopher, Gould, Trevor J, Wang, Ping, Phillips, Jane, Cotner, James B, Sadowsky, Michael J
Format:	Artikel
Sprache:	eng
Schlagworte:	Ammonium Ammonium compounds Ampicillin Antibiotic resistance Antibiotics Antimicrobial agents Artificial chromosomes Bacteria Bacteria - genetics Biofilms Cephalothin Chemical contaminants Clones Communities Community composition Correlation Deoxyribonucleic acid Disease resistance DNA DNA, Bacterial - analysis DNA, Bacterial - genetics Drug resistance Drug Resistance, Bacterial - genetics Freshwater Genes Genes, Bacterial - genetics High-Throughput Nucleotide Sequencing Kanamycin Land cover Low frequencies Metagenomics Mineral nutrients Mississippi Nutrient concentrations Pathogens Reservoirs River ecology Rivers Rivers - microbiology Screening Sequence Analysis, DNA Studies Total dissolved solids Water Microbiology
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Zusammenfassung:	In this study, we determined the frequency of antibiotic resistance genes (ARGs) in the Upper Mississippi River using a high-throughput, functional, metagenomic screening procedure. Fosmid libraries containing ∼10,000 clones were screened for resistance to ampicillin, cephalothin, kanamycin, and tetracycline. We hypothesized that nutrient concentrations, land cover type, and taxonomic community composition may select for ARGs. Resistance to ampicillin, cephalothin, and kanamycin was low (
ISSN:	1477-8920 1996-7829
DOI:	10.2166/wh.2014.215