Application of micro morr E-3360 as bioremediation material for marine oil spill

This research was aimed to test the feasibility of Micro Morr E-3360 as bioremediation material for marine oil spill at laboratory scale. Feasibility was based on its effectiveness to decrease Total Petroleum Hydrocarbon (TPH) content, toxicity of seawater sample after bioremediation and its immobilization media residue. TPH content analysis was conducted using UV- Vis spectrophotometry method and toxicity was measured using Brine Shrimp Lethality Test (BSL T) method. Identification of the bacterial consortium in the bioremediation media was conducted using 16 S rDNA gene sequencing analysis. Research was conducted in five experiments in beakers which are seawater and oil 2% (w/w) of seawater (M); seawater, oil 2% (w/w) of seawater, bioremediant 10% (w/w) of oil, and nutrient (P); seawater as control (AL); seawater and bioremediant 10% (w/w) of oil (B) and seawater and nutrient (K). Identification result showed that bacteria consortium consisted of three species, they were Bacillus licheniformis, B. thermoamylovorans and Geobacillus pallidus. Bioremediant was able to decrease TPH concentration in sea water by 77% and toxicity level by 88% in 20 days. Oil content in immobilization media residue was also low, that was 2 ppm. However, significant oxygen depletion and turbidity increment could be the principal factor causing 50% lethality of A. salina population.Original Abstract: Penelitian ini bertujuan untuk menguji kelayakan Micro Morr E-3360 sebagai bahan bioremedian tumpahan minyak dilaut pada skala laboratorium. Kelayakan didasari pada efektivitas pengurangan kadar Total Petroleum Hydrocarbon (TPH), toksisitas air laut hasil bioremediasi dan residu media imobilisasi dari produk. Analisis kadar TPH dilakukan dengan menggunakan spektrofotometer UV-Vis dan tingkat toksisitas diukur menggunakan metode Brine Shrimp Lethality Test (8SL T). Identifikasi konsorsium bakteri dilakukan pada media imobilisasi dengan analisis sekuens gen 16S rDNA. Penelitian dilakukan dengan lima uji coba dalam beaker, yaitu air laut dan minyak 2% w/w dari air laut (M); air laut, minyak 2% w/w dari air laut, bioremedian 10% w/w dari minyak, dan nutrien (P); kontrol air laut (AL); kontrol air laut dan bioremedian 10% w/w dari minyak (8); serta air laut dan nutrien (K). Hasil analisis menunjukkan bahwa konsorsium bakteri terdiri dari 3 spesies bakteri, yaitu Bacillus licheniformis, B. thermoamylovorans, dan Geobacillus pallidus. Bioremedian ini mampu menurunkan kadar TPH dalam air seb