Urine Nested Polymerase Chain Reaction in Neonatal Septicemia

This cross-sectional study was done to evaluate diagnostic efficacy of urine nested polymerase chain reaction (PCR) using broad-range 16SrDNA PCR-based amplification, followed by restriction analysis and sequencing in neonatal septicemia. The study included 50 babies; 48% had vaginal delivery, 46% w...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of tropical pediatrics (1980) 2015-08, Vol.61 (4), p.295-300
Hauptverfasser:	Das, B K, Suri, Shipra, Nath, Gopal, Prasad, Rajniti
Format:	Artikel
Sprache:	eng
Schlagworte:	C-Reactive Protein - analysis Cross-Sectional Studies DNA - analysis DNA, Bacterial Female Genes, rRNA - genetics Humans Infant, Newborn Klebsiella pneumoniae Klebsiella pneumoniae - isolation & purification Leukocyte Count Polymerase Chain Reaction - methods Predictive Value of Tests RNA, Ribosomal, 16S - analysis Sensitivity and Specificity Sepsis - diagnosis Sepsis - microbiology Sepsis - urine Sequence Analysis, DNA Staphylococcus aureus - isolation & purification Urine - microbiology
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	This cross-sectional study was done to evaluate diagnostic efficacy of urine nested polymerase chain reaction (PCR) using broad-range 16SrDNA PCR-based amplification, followed by restriction analysis and sequencing in neonatal septicemia. The study included 50 babies; 48% had vaginal delivery, 46% were preterm, 20% had a history of prolonged rupture of membranes and 56% were low birth weight (≤2500 g). Clinical presentations were lethargy (96%), respiratory distress (80%) and bleeding diathesis (16%). Absolute neutrophil count
ISSN:	0142-6338 1465-3664
DOI:	10.1093/tropej/fmv043