Enhanced production of α-amylase by Penicillium chrysogenum in liquid culture by modifying the process parameters

In this paper, we have assessed the role of changing physicochemical parameters and substrate types on the production of α-amylase enzyme from Penicillium chrysogenum, with a view to determining the optimal conditions required for its maximum production. The findings of this research revealed that, at pH 6 using linseed oil cake as substratum, α-amylase enzyme production was maximum (550.0 U/mL), when the fungi was incubated for 6 days at 30 °C in 0.1 M acetate buffer. Further, reasonably good production of the α-amylase enzyme was also observed at pH 9 with all the experimented carbon sources as substrates. Moreover, statistical analysis, using analysis of variance (ANOVA) carried out to study the impact of different studied parameters on the α-amylase enzyme production revealed that incubation period of 6–18 days is highly significant (p = 0.01) factor in amylotic activity of the P. chrysogenum. Under the researched out optimal conditions, P. chrysogenum is an economically viable option for the industrial and biotechnological production of α-amylase enzyme. •Fungus Penicillium chrysogenum was isolated from the soil and the α-amylase production from it by the fermentation process was studied.•Findings have revealed that, at pH 6 using linseed oil cake as substratum, α-amylase enzyme production was maximum.•Statistical analysis of α-amylase enzyme production revealed that incubation period of 6–18 days was highly significant (p = 0.01).•Pencillium chrysogenum can be economically a viable option for the industrial and biotechnological production of α-amylase.