Eight new equine dinucleotide repeat microsatellites at the NVHEQ26, NVHEQ29, NVHEQ31, NVHEQ40, NVHEQ43, NVHEQ90, NVHEQ98, and NVHEQ100 loci

Source/description: Equine genomic DNA was digested with Sau3AI and size selected fragments (300-600 bp) were ligated into the BamHI site of the BluescriptSK + plasmid. The library was screened with a synthetic (GT) sub(10) oligonucleotide end-labeled with gamma super(32)P ATP. Positive clones were picked and rescreened and the double positives were sequenced with dye terminator chemistry on an ABI 310 sequencer. The sequences have been submitted to the GenBank database (accession numbers are listed in Table 1). Primers for the PCR were designed to amplify the regions containing the dinucleotide repeats (Table 1). Mendelian inheritance: Autosomal codominant segregation was demonstrated in a Danish fullsib mink pedigree. Polymorphism: Studies for variation were performed in five populations of unrelated farm mink; two lines of Scanblack, two lines of Royal Pastel and one line of Standard mink `Wild' (Table 1). The size of the alleles were scored using a 50-500 bp ladder (Pharmacia) together with the amplified isolated clone as an external size marker.