Multilayered, Hyaluronic Acid-Based Hydrogel Formulations Suitable for Automated 3D High Throughput Drug Screening of Cancer-Stromal Cell Cocultures

Validation of a high‐throughput compatible 3D hyaluronic acid hydrogel coculture of cancer cells with stromal cells. The multilayered hyaluronic acid hydrogels improve drug screening predictability as evaluated with a panel of clinically relevant chemotherapeutics in both prostate and endometrial ca...

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Veröffentlicht in:	Advanced healthcare materials 2015-08, Vol.4 (11), p.1664-1674
Hauptverfasser:	Engel, Brian J., Constantinou, Pamela E., Sablatura, Lindsey K., Doty, Nathaniel J., Carson, Daniel D., Farach-Carson, Mary C., Harrington, Daniel A., Zarembinski, Thomas I.
Format:	Artikel
Sprache:	eng
Schlagworte:	3D culture Antineoplastic Agents - chemistry Antineoplastic Agents - toxicity Automation Biotechnology Cancer Cell Culture Techniques Cell Line, Tumor Cell Survival - drug effects coculture Coculture Techniques Culture Drug Evaluation, Preclinical drug screening Drugs high throughput High-Throughput Screening Assays - instrumentation High-Throughput Screening Assays - methods Humans Hyaluronic acid Hyaluronic Acid - chemistry Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry Hydrogels Microscopy, Confocal Screening Stromal Cells - cytology Stromal Cells - metabolism Three dimensional
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Zusammenfassung:	Validation of a high‐throughput compatible 3D hyaluronic acid hydrogel coculture of cancer cells with stromal cells. The multilayered hyaluronic acid hydrogels improve drug screening predictability as evaluated with a panel of clinically relevant chemotherapeutics in both prostate and endometrial cancer cell lines compared to 2D culture.
ISSN:	2192-2640 2192-2659
DOI:	10.1002/adhm.201500258