Detecting miRNA by producing RNA: a sensitive assay that combines rolling-circle DNA polymerization and rolling circle transcription

Detecting miRNA by producing RNA: a sensitive assay that combines rolling-circle DNA polymerization and rolling circle transcription

Target miRNA was detected by producing RNA: rolling circle polymerization (RCP) and rolling circle transcription (RCT) were interlinked to provide dual amplification, producing multiplied malachite green (MG) aptamers, and a signal was generated by the SERS (surface-enhanced Raman scattering) quanti...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2015-08, Vol.51 (60), p.11976-11979
Hauptverfasser: Li, Xuemei, Zheng, Fuwei, Ren, Rui
Format: Artikel
Sprache:eng
Schlagworte:
Amplification
Aptamers, Nucleotide - chemistry
Aptamers, Nucleotide - genetics
Assaying
Deoxyribonucleic acid
DNA, Circular - chemistry
DNA, Circular - genetics
HeLa Cells
Humans
Malachite green
MicroRNAs - analysis
MicroRNAs - genetics
Nucleic Acid Amplification Techniques - methods
Polymerization
Raman scattering
Ribonucleic acids
Rosaniline Dyes - chemistry
Spectrum Analysis, Raman - methods
Target detection
Transcription, Genetic
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Zusammenfassung:Target miRNA was detected by producing RNA: rolling circle polymerization (RCP) and rolling circle transcription (RCT) were interlinked to provide dual amplification, producing multiplied malachite green (MG) aptamers, and a signal was generated by the SERS (surface-enhanced Raman scattering) quantification of the MG molecules that were bound to the transcripts.
ISSN:1359-7345
1364-548X
DOI:10.1039/c5cc01748c