Quencher-free molecular aptamer beacons (QF-MABs) for detection of ATP

We have developed a simple and efficient system—based on quencher-free molecular aptamer beacons (QF-MABs)—for probing ATP. In the absence of ATP, the fluorescence of a pyrene fluorophore on the loop position of the optimal QF-MAB was quenched by the neighboring nucleobases; in its presence, fluorescence was recovered, due to a conformational change in the secondary structure of the QF-MAB. [Display omitted] We have constructed a simple and efficient system—based on quencher-free molecular aptamer beacons (QF-MABs)—for probing ATP. In the absence of ATP, the fluorescence of a pyrene fluorophore on the loop position (15 nucleotides from the 5′ end) of the optimal QF-MAB was quenched by the neighboring nucleobases; in its presence, fluorescence was recovered, due to a conformational change in the secondary structure of the QF-MAB.