A new method for ABO genotyping using fluorescence melting curve analysis based on peptide nucleic acid probes

Highlights • We developed PNA probe-based FMCA for ABO genotyping. • This method allowed for simultaneous detection of three SNP sites in the ABO gene. • Eighty samples were successfully genotyped. • Reactions were performed in one single closed tube in less than 3 h. • This method may be an effecti...

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Veröffentlicht in:	Legal medicine (Tokyo, Japan) Japan), 2015-09, Vol.17 (5), p.334-339
Hauptverfasser:	Lee, Kyungmyung, Park, Hyun-Chul, An, Sanghyun, Ahn, Eu-Ree, Lee, Yang-Han, Kim, Mi-Jung, Lee, Eun-jung, Park, Jae Sin, Jung, Jin Wook, Lim, Sikeun
Format:	Artikel
Sprache:	eng
Schlagworte:	ABO Blood-Group System - genetics ABO genotyping Crime Fluorescence Fluorescence melting curve analysis Forensic Medicine - methods Genotyping Techniques - methods Humans Hybridization, Genetic Internal Medicine Peptide nucleic acid Peptide Nucleic Acids - analysis Polymerase Chain Reaction Polymorphism, Single Nucleotide Real-Time Polymerase Chain Reaction Single nucleotide polymorphism
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Zusammenfassung:	Highlights • We developed PNA probe-based FMCA for ABO genotyping. • This method allowed for simultaneous detection of three SNP sites in the ABO gene. • Eighty samples were successfully genotyped. • Reactions were performed in one single closed tube in less than 3 h. • This method may be an effective and helpful tool in forensic investigations.
ISSN:	1344-6223 1873-4162
DOI:	10.1016/j.legalmed.2015.04.001