The structure-forming role of magnesium pyrophosphate in the formation of DNA-containing microparticles during PCR
This work is devoted to studying the mechanisms of formation of DNA-containing microparticles (MPs) during PCR. It was found that pyrophosphate, a byproduct of DNA synthesis, and magnesium cations are required for their formation, as evidenced by the results of biochemical and electron microscopy st...
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| Veröffentlicht in: | Doklady. Biochemistry and biophysics 2015-07, Vol.463 (1), p.259-263 |
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| container_issue | 1 |
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| container_title | Doklady. Biochemistry and biophysics |
| container_volume | 463 |
| creator | Danilevich, V. N. Machulin, A. V. Sorokin, V. V. Mulyukin, A. L. |
| description | This work is devoted to studying the mechanisms of formation of DNA-containing microparticles (MPs) during PCR. It was found that pyrophosphate, a byproduct of DNA synthesis, and magnesium cations are required for their formation, as evidenced by the results of biochemical and electron microscopy studies. |
| doi_str_mv | 10.1134/S1607672915040171 |
| format | Article |
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| ispartof | Doklady. Biochemistry and biophysics, 2015-07, Vol.463 (1), p.259-263 |
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| subjects | Biochemistry Biological and Medical Physics Biomedical and Life Sciences Biophysics Biophysics and Molecular Biology Diphosphates - metabolism Diphosphates - pharmacology DNA - chemistry DNA - genetics DNA - metabolism Life Sciences Magnesium Chloride - pharmacology Magnesium Compounds - metabolism Magnesium Compounds - pharmacology Microspheres Polymerase Chain Reaction |
| title | The structure-forming role of magnesium pyrophosphate in the formation of DNA-containing microparticles during PCR |
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