The structure-forming role of magnesium pyrophosphate in the formation of DNA-containing microparticles during PCR

This work is devoted to studying the mechanisms of formation of DNA-containing microparticles (MPs) during PCR. It was found that pyrophosphate, a byproduct of DNA synthesis, and magnesium cations are required for their formation, as evidenced by the results of biochemical and electron microscopy st...

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Veröffentlicht in:	Doklady. Biochemistry and biophysics 2015-07, Vol.463 (1), p.259-263
Hauptverfasser:	Danilevich, V. N., Machulin, A. V., Sorokin, V. V., Mulyukin, A. L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biochemistry Biological and Medical Physics Biomedical and Life Sciences Biophysics Biophysics and Molecular Biology Diphosphates - metabolism Diphosphates - pharmacology DNA - chemistry DNA - genetics DNA - metabolism Life Sciences Magnesium Chloride - pharmacology Magnesium Compounds - metabolism Magnesium Compounds - pharmacology Microspheres Polymerase Chain Reaction
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Beschreibung
Zusammenfassung:	This work is devoted to studying the mechanisms of formation of DNA-containing microparticles (MPs) during PCR. It was found that pyrophosphate, a byproduct of DNA synthesis, and magnesium cations are required for their formation, as evidenced by the results of biochemical and electron microscopy studies.
ISSN:	1607-6729 1608-3091
DOI:	10.1134/S1607672915040171