Burkholderia metalliresistens sp. nov., a multiple metal-resistant and phosphate-solubilising species isolated from heavy metal-polluted soil in Southeast China

A metal-resistant and phosphate-solubilising bacterium, designated as strain D414 T , was isolated from heavy metal (Pb, Cd, Cu and Zn)-polluted paddy soils at the surrounding area of Dabao Mountain Mine in Southeast China. The minimum inhibitory concentrations of heavy metals for strain D414 T were 2000 mg L −1 (Cd), 800 mg L −1 (Pb), 150 mg L −1 (Cu) and 2500 mg L −1 (Zn). The strain possessed plant growth-promoting properties, such as 1-aminocyclopropane-1-carboxylate assimilation, indole production and phosphate solubilisation. Analysis of 16S rRNA gene sequence indicated that the isolate is a member of the genus Burkholderia where strain D414 T formed a distinct phyletic line with validly described Burkholderia species. Strain D414 T is closely related to Burkholderia tropica DSM 15359 T , B. bannensis NBRC E25 T and B. unamae DSM 17197 T , with 98.5, 98.3 and 98.3 % sequence similarities, respectively. Furthermore, less than 34 % DNA–DNA relatedness was detected between strain D414 T and the type strains of the phylogenetically closest species of Burkholderia . The dominant fatty acids of strain D414 T were C 14:0 , C 16:0 , C 17:0 cyclo and C 18:1 ω 7 c . The DNA G+C content was 62.3 ± 0.5 mol%. On the basis of genotypic, phenotypic and phylogenetic data, strain D414 T represents a novel species, for which the name Burkholderia metalliresistens sp. nov. is proposed, with D414 T (=CICC 10561 T = DSM 26823 T ) as the type strain