Protein Synthesis in Sub-Micrometer Water-in-Oil Droplets

Water‐in‐oil (w/o) emulsions are used as a cellular model because of their unique cell‐like architecture. Previous works showed the capability of eukaryotic‐cell‐sized w/o droplets (5–50 μm) to support protein synthesis efficiently; however data about smaller w/o compartments (

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Bibliographische Detailangaben
Veröffentlicht in:	Chembiochem : a European journal of chemical biology 2015-09, Vol.16 (14), p.2073-2079
Hauptverfasser:	Gallo, Valentina, Stano, Pasquale, Luisi, Pier Luigi
Format:	Artikel
Sprache:	eng
Schlagworte:	cell-free protein synthesis Cell-Free System - metabolism Cholesterol - metabolism Emulsions - chemistry Escherichia coli - metabolism Green Fluorescent Proteins - metabolism Hexanols - chemistry Lecithins - chemistry microcompartments Oils - chemistry Protein Biosynthesis solvent effects Surface-Active Agents - chemistry surfactants Water - chemistry water-in-oil emulsions
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Beschreibung
Zusammenfassung:	Water‐in‐oil (w/o) emulsions are used as a cellular model because of their unique cell‐like architecture. Previous works showed the capability of eukaryotic‐cell‐sized w/o droplets (5–50 μm) to support protein synthesis efficiently; however data about smaller w/o compartments (
ISSN:	1439-4227 1439-7633
DOI:	10.1002/cbic.201500274