Sorbitol-mediated stabilization of human IgG against thermal inactivation

Sorbitol at 30% (w/w) stabilized human IgG to thermal denaturation from 60 to 85 degree C, increasing the protein's half life from 25 to 266 min at 70 degree C. A kinetic model based on the Lumry-Eyring inactivation scheme was developed and used to estimate the apparent rate constant and activa...

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Veröffentlicht in:	Biotechnology techniques 1998-11, Vol.12 (11), p.855-858
Hauptverfasser:	CIOCI, F, LAVECCHIA, R
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Others Various methods and equipments
Online-Zugang:	Volltext
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Zusammenfassung:	Sorbitol at 30% (w/w) stabilized human IgG to thermal denaturation from 60 to 85 degree C, increasing the protein's half life from 25 to 266 min at 70 degree C. A kinetic model based on the Lumry-Eyring inactivation scheme was developed and used to estimate the apparent rate constant and activation energy.
ISSN:	0951-208X 1573-6784
DOI:	10.1023/A:1008839912640