Reversible inactivation of nitrogenase in Rhodobacter capsulatus strain W107I deleted in the draTG gene region

Reversible inactivation of nitrogenase in Rhodobacter capsulatus strain W107I deleted in the draTG gene region

Abstract Upon addition of ammonium or removal of light, nitrogenase was reversibly inactivated in a draTG gene deletion mutant of Rhodobacter capsulatus. Reversible inactivation by exposure of the mutant to darkness followed the same kinetics as in control strains containing the draTG gene region. R...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:FEMS microbiology letters 1999-01, Vol.170 (1), p.167-171
Hauptverfasser: Förster, Beate, Maner, Klaus, Fassbinder, Frank, Oelze, Jürgen
Format: Artikel
Sprache:eng
Schlagworte:
Activation
Adenosine diphosphate
ADP-ribosylation
Ammonium
Continuous culture
Darkness
Deactivation
Deletion mutant
Gene deletion
Inactivation
Microbiology
Nitrogenase
Nitrogenase inactivation
Reaction kinetics
Rhodobacter capsulatus
Ribose
Ribosylation
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Abstract Upon addition of ammonium or removal of light, nitrogenase was reversibly inactivated in a draTG gene deletion mutant of Rhodobacter capsulatus. Reversible inactivation by exposure of the mutant to darkness followed the same kinetics as in control strains containing the draTG gene region. Reactivation of nitrogenase by light was significantly faster than removal of ADP-ribose from component 2 of nitrogenase. The results show that, in R capsulatus, reversible inactivation of nitrogenase is independent of reversible ADP-ribosylation by the DRAT/DRAG enzyme system encoded by the draTG gene region.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1999.tb13370.x