Identification of a lactose-responsive element upstream of the promoter of Bacillus megaterium beta-galactosidase-encoding gene mbgA

The Bacillus megaterium mbgA gene encodes a lactose-hydrolyzing beta-galactosidase. An AraC/XylS-type activator BgaR can activate mbgA transcription in response to lactose. In this report, we show by various deletion analyses and point mutagenesis analyses that an inverted repeat centered at positio...

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Veröffentlicht in:	Current microbiology 2005-07, Vol.51 (1), p.31-34
Hauptverfasser:	Li, Jen-Ming, Chiou, Chih-Yung, Lee, Tian-Ren, Chen, Yuan-Shou, Shaw, Gwo-Chyuan
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacillus megaterium Bacillus megaterium - enzymology Bacillus megaterium - genetics Bacillus megaterium - growth & development Base Sequence beta-Galactosidase - genetics beta-Galactosidase - metabolism DNA, Bacterial - genetics DNA, Bacterial - metabolism Gene Expression Regulation, Bacterial Genome, Bacterial Lac Operon Lactose - metabolism Lactose - physiology Molecular Sequence Data Promoter Regions, Genetic
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Zusammenfassung:	The Bacillus megaterium mbgA gene encodes a lactose-hydrolyzing beta-galactosidase. An AraC/XylS-type activator BgaR can activate mbgA transcription in response to lactose. In this report, we show by various deletion analyses and point mutagenesis analyses that an inverted repeat centered at position -60.5 relative to the mbgA transcriptional initiation site is the cis-acting element responsible for lactose induction of mbgA expression.
ISSN:	0343-8651 1432-0991
DOI:	10.1007/s00284-005-4495-8