Electrophysiological effects of Bacillus sphaericus binary toxin on cultured mosquito cells
The electrophysiological effects of both trypsin-activated native and 42- and 51-kDa cloned binary toxins of Bacillus sphaericus were investigated on cultured Culex quinquefasciatus cells using the patch-clamp technique. Rates of reduction in whole-cell membrane resistance were correlated with incre...
Gespeichert in:
| Veröffentlicht in: | Journal of invertebrate pathology 1997-05, Vol.69 (3), p.197-204 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 204 |
|---|---|
| container_issue | 3 |
| container_start_page | 197 |
| container_title | Journal of invertebrate pathology |
| container_volume | 69 |
| creator | COKMUS, C DAVIDSON, E. W COOPER, K |
| description | The electrophysiological effects of both trypsin-activated native and 42- and 51-kDa cloned binary toxins of Bacillus sphaericus were investigated on cultured Culex quinquefasciatus cells using the patch-clamp technique. Rates of reduction in whole-cell membrane resistance were correlated with increasing native toxin concentration. The 42- or 51-kDa cloned toxin alone at 50 micrograms/ml reduced the resistance. Electrophysiological effects occurred before any changes were visible by phase-contrast microscopy. |
| doi_str_mv | 10.1006/jipa.1997.4660 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_17119945</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>17119945</sourcerecordid><originalsourceid>FETCH-LOGICAL-f221t-c1707367498e3840c890481ef4de10fa71e5f3e3504ed5b49212c90aa8c05fd53</originalsourceid><addsrcrecordid>eNo9kbtPwzAQxi0EKqWwsoE8ILaU8yMPj1CVh1SJAToxRK5jt67cOI0Tif73uGrEdJ_u--l09x1CtwSmBCB72tpGTokQ-ZRnGZyhMQGRJVBAeo7GAJQmFAi5RFchbCGqNBMjNBIkB8bTMfqZO6261jebQ7De-bVV0mFtTOwG7A1-kco61wccmo3UrVVRrmwt2wPu_K-tsa-x6l3Xt7rCOx_2ve08Vtq5cI0ujHRB3wx1gpav8-_Ze7L4fPuYPS8SQynpEhV3yVmWc1FoVnBQhQBeEG14pQkYmROdGqZZClxX6YoLSqgSIGWhIDVVyibo8TS3af2-16ErdzYcN5C19n0oSU5iQPwI3g1gv9rpqmxau4uHlEMa0X8YfBliDKaVtbLhH6M5ybhgEbs_YUb6Uq7biCy_ji-A6APP2B9X63gu</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17119945</pqid></control><display><type>article</type><title>Electrophysiological effects of Bacillus sphaericus binary toxin on cultured mosquito cells</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>COKMUS, C ; DAVIDSON, E. W ; COOPER, K</creator><creatorcontrib>COKMUS, C ; DAVIDSON, E. W ; COOPER, K ; Concepcion Univ. (Chile). Vicerrectoria Academica ; Ankara University, Turkey</creatorcontrib><description>The electrophysiological effects of both trypsin-activated native and 42- and 51-kDa cloned binary toxins of Bacillus sphaericus were investigated on cultured Culex quinquefasciatus cells using the patch-clamp technique. Rates of reduction in whole-cell membrane resistance were correlated with increasing native toxin concentration. The 42- or 51-kDa cloned toxin alone at 50 micrograms/ml reduced the resistance. Electrophysiological effects occurred before any changes were visible by phase-contrast microscopy.</description><identifier>ISSN: 0022-2011</identifier><identifier>EISSN: 1096-0805</identifier><identifier>DOI: 10.1006/jipa.1997.4660</identifier><identifier>PMID: 9170345</identifier><identifier>CODEN: JIVPAZ</identifier><language>eng</language><publisher>Amsterdam: Elsevier</publisher><subject>Animals ; Bacillus ; Bacillus sphaericus ; bacterial toxins ; Bacterial Toxins - toxicity ; Biological and medical sciences ; cell culture ; Cell Membrane - drug effects ; Cell Membrane - physiology ; cell membranes ; Cells, Cultured ; Culex - drug effects ; Culex quinquefasciatus ; Culicidae ; cultivo de celulas ; culture de cellule ; Electrophysiology ; Fundamental and applied biological sciences. Psychology ; Insecticides - toxicity ; Medically important nuisances and vectors, pests of stored products and materials: population survey and control ; membranas celulares ; membrane cellulaire ; Patch-Clamp Techniques ; toxicidad ; toxicite ; toxicity ; toxinas bacterianas ; toxine bacterienne ; Vectors. Intermediate hosts</subject><ispartof>Journal of invertebrate pathology, 1997-05, Vol.69 (3), p.197-204</ispartof><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2716493$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9170345$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>COKMUS, C</creatorcontrib><creatorcontrib>DAVIDSON, E. W</creatorcontrib><creatorcontrib>COOPER, K</creatorcontrib><creatorcontrib>Concepcion Univ. (Chile). Vicerrectoria Academica</creatorcontrib><creatorcontrib>Ankara University, Turkey</creatorcontrib><title>Electrophysiological effects of Bacillus sphaericus binary toxin on cultured mosquito cells</title><title>Journal of invertebrate pathology</title><addtitle>J Invertebr Pathol</addtitle><description>The electrophysiological effects of both trypsin-activated native and 42- and 51-kDa cloned binary toxins of Bacillus sphaericus were investigated on cultured Culex quinquefasciatus cells using the patch-clamp technique. Rates of reduction in whole-cell membrane resistance were correlated with increasing native toxin concentration. The 42- or 51-kDa cloned toxin alone at 50 micrograms/ml reduced the resistance. Electrophysiological effects occurred before any changes were visible by phase-contrast microscopy.</description><subject>Animals</subject><subject>Bacillus</subject><subject>Bacillus sphaericus</subject><subject>bacterial toxins</subject><subject>Bacterial Toxins - toxicity</subject><subject>Biological and medical sciences</subject><subject>cell culture</subject><subject>Cell Membrane - drug effects</subject><subject>Cell Membrane - physiology</subject><subject>cell membranes</subject><subject>Cells, Cultured</subject><subject>Culex - drug effects</subject><subject>Culex quinquefasciatus</subject><subject>Culicidae</subject><subject>cultivo de celulas</subject><subject>culture de cellule</subject><subject>Electrophysiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Insecticides - toxicity</subject><subject>Medically important nuisances and vectors, pests of stored products and materials: population survey and control</subject><subject>membranas celulares</subject><subject>membrane cellulaire</subject><subject>Patch-Clamp Techniques</subject><subject>toxicidad</subject><subject>toxicite</subject><subject>toxicity</subject><subject>toxinas bacterianas</subject><subject>toxine bacterienne</subject><subject>Vectors. Intermediate hosts</subject><issn>0022-2011</issn><issn>1096-0805</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kbtPwzAQxi0EKqWwsoE8ILaU8yMPj1CVh1SJAToxRK5jt67cOI0Tif73uGrEdJ_u--l09x1CtwSmBCB72tpGTokQ-ZRnGZyhMQGRJVBAeo7GAJQmFAi5RFchbCGqNBMjNBIkB8bTMfqZO6261jebQ7De-bVV0mFtTOwG7A1-kco61wccmo3UrVVRrmwt2wPu_K-tsa-x6l3Xt7rCOx_2ve08Vtq5cI0ujHRB3wx1gpav8-_Ze7L4fPuYPS8SQynpEhV3yVmWc1FoVnBQhQBeEG14pQkYmROdGqZZClxX6YoLSqgSIGWhIDVVyibo8TS3af2-16ErdzYcN5C19n0oSU5iQPwI3g1gv9rpqmxau4uHlEMa0X8YfBliDKaVtbLhH6M5ybhgEbs_YUb6Uq7biCy_ji-A6APP2B9X63gu</recordid><startdate>19970501</startdate><enddate>19970501</enddate><creator>COKMUS, C</creator><creator>DAVIDSON, E. W</creator><creator>COOPER, K</creator><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7SS</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>19970501</creationdate><title>Electrophysiological effects of Bacillus sphaericus binary toxin on cultured mosquito cells</title><author>COKMUS, C ; DAVIDSON, E. W ; COOPER, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f221t-c1707367498e3840c890481ef4de10fa71e5f3e3504ed5b49212c90aa8c05fd53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Bacillus</topic><topic>Bacillus sphaericus</topic><topic>bacterial toxins</topic><topic>Bacterial Toxins - toxicity</topic><topic>Biological and medical sciences</topic><topic>cell culture</topic><topic>Cell Membrane - drug effects</topic><topic>Cell Membrane - physiology</topic><topic>cell membranes</topic><topic>Cells, Cultured</topic><topic>Culex - drug effects</topic><topic>Culex quinquefasciatus</topic><topic>Culicidae</topic><topic>cultivo de celulas</topic><topic>culture de cellule</topic><topic>Electrophysiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Insecticides - toxicity</topic><topic>Medically important nuisances and vectors, pests of stored products and materials: population survey and control</topic><topic>membranas celulares</topic><topic>membrane cellulaire</topic><topic>Patch-Clamp Techniques</topic><topic>toxicidad</topic><topic>toxicite</topic><topic>toxicity</topic><topic>toxinas bacterianas</topic><topic>toxine bacterienne</topic><topic>Vectors. Intermediate hosts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>COKMUS, C</creatorcontrib><creatorcontrib>DAVIDSON, E. W</creatorcontrib><creatorcontrib>COOPER, K</creatorcontrib><creatorcontrib>Concepcion Univ. (Chile). Vicerrectoria Academica</creatorcontrib><creatorcontrib>Ankara University, Turkey</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of invertebrate pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>COKMUS, C</au><au>DAVIDSON, E. W</au><au>COOPER, K</au><aucorp>Concepcion Univ. (Chile). Vicerrectoria Academica</aucorp><aucorp>Ankara University, Turkey</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electrophysiological effects of Bacillus sphaericus binary toxin on cultured mosquito cells</atitle><jtitle>Journal of invertebrate pathology</jtitle><addtitle>J Invertebr Pathol</addtitle><date>1997-05-01</date><risdate>1997</risdate><volume>69</volume><issue>3</issue><spage>197</spage><epage>204</epage><pages>197-204</pages><issn>0022-2011</issn><eissn>1096-0805</eissn><coden>JIVPAZ</coden><abstract>The electrophysiological effects of both trypsin-activated native and 42- and 51-kDa cloned binary toxins of Bacillus sphaericus were investigated on cultured Culex quinquefasciatus cells using the patch-clamp technique. Rates of reduction in whole-cell membrane resistance were correlated with increasing native toxin concentration. The 42- or 51-kDa cloned toxin alone at 50 micrograms/ml reduced the resistance. Electrophysiological effects occurred before any changes were visible by phase-contrast microscopy.</abstract><cop>Amsterdam</cop><pub>Elsevier</pub><pmid>9170345</pmid><doi>10.1006/jipa.1997.4660</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-2011 |
| ispartof | Journal of invertebrate pathology, 1997-05, Vol.69 (3), p.197-204 |
| issn | 0022-2011 1096-0805 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_17119945 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Bacillus Bacillus sphaericus bacterial toxins Bacterial Toxins - toxicity Biological and medical sciences cell culture Cell Membrane - drug effects Cell Membrane - physiology cell membranes Cells, Cultured Culex - drug effects Culex quinquefasciatus Culicidae cultivo de celulas culture de cellule Electrophysiology Fundamental and applied biological sciences. Psychology Insecticides - toxicity Medically important nuisances and vectors, pests of stored products and materials: population survey and control membranas celulares membrane cellulaire Patch-Clamp Techniques toxicidad toxicite toxicity toxinas bacterianas toxine bacterienne Vectors. Intermediate hosts |
| title | Electrophysiological effects of Bacillus sphaericus binary toxin on cultured mosquito cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T18%3A58%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Electrophysiological%20effects%20of%20Bacillus%20sphaericus%20binary%20toxin%20on%20cultured%20mosquito%20cells&rft.jtitle=Journal%20of%20invertebrate%20pathology&rft.au=COKMUS,%20C&rft.aucorp=Concepcion%20Univ.%20(Chile).%20Vicerrectoria%20Academica&rft.date=1997-05-01&rft.volume=69&rft.issue=3&rft.spage=197&rft.epage=204&rft.pages=197-204&rft.issn=0022-2011&rft.eissn=1096-0805&rft.coden=JIVPAZ&rft_id=info:doi/10.1006/jipa.1997.4660&rft_dat=%3Cproquest_pubme%3E17119945%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17119945&rft_id=info:pmid/9170345&rfr_iscdi=true |