Electrophysiological effects of Bacillus sphaericus binary toxin on cultured mosquito cells

The electrophysiological effects of both trypsin-activated native and 42- and 51-kDa cloned binary toxins of Bacillus sphaericus were investigated on cultured Culex quinquefasciatus cells using the patch-clamp technique. Rates of reduction in whole-cell membrane resistance were correlated with incre...

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Veröffentlicht in:	Journal of invertebrate pathology 1997-05, Vol.69 (3), p.197-204
Hauptverfasser:	COKMUS, C, DAVIDSON, E. W, COOPER, K
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacillus Bacillus sphaericus bacterial toxins Bacterial Toxins - toxicity Biological and medical sciences cell culture Cell Membrane - drug effects Cell Membrane - physiology cell membranes Cells, Cultured Culex - drug effects Culex quinquefasciatus Culicidae cultivo de celulas culture de cellule Electrophysiology Fundamental and applied biological sciences. Psychology Insecticides - toxicity Medically important nuisances and vectors, pests of stored products and materials: population survey and control membranas celulares membrane cellulaire Patch-Clamp Techniques toxicidad toxicite toxicity toxinas bacterianas toxine bacterienne Vectors. Intermediate hosts
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Zusammenfassung:	The electrophysiological effects of both trypsin-activated native and 42- and 51-kDa cloned binary toxins of Bacillus sphaericus were investigated on cultured Culex quinquefasciatus cells using the patch-clamp technique. Rates of reduction in whole-cell membrane resistance were correlated with increasing native toxin concentration. The 42- or 51-kDa cloned toxin alone at 50 micrograms/ml reduced the resistance. Electrophysiological effects occurred before any changes were visible by phase-contrast microscopy.
ISSN:	0022-2011 1096-0805
DOI:	10.1006/jipa.1997.4660