Antimicrobial effect of photodynamic therapy in carious lesions in vivo , using culture and real-time PCR methods

Highlights • We evaluated the antimicrobial effect of PDT in carious lesions in vivo. • We used methylene blue at 100 mg/L associated with a halogen light source. • Viable bacteria were significantly reduced in all of the agar plates after PDT. • The number of Streptococcus mutans DNA copies were no...

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Veröffentlicht in:	Photodiagnosis and photodynamic therapy 2015-09, Vol.12 (3), p.401-407
Hauptverfasser:	Araújo, Patricia Valente, Correia-Silva, Jeane de Fátima, Gomez, Ricardo Santiago, Massara, Maria de Lourdes de Andrade, Cortes, Maria Esperanza, Poletto, Luiz Thadeu de Abreu
Format:	Artikel
Sprache:	eng
Schlagworte:	Caries Child Child, Preschool Dental Caries - drug therapy Dentin DNA, Bacterial Female Hematology, Oncology and Palliative Medicine Humans Internal Medicine Male Methylene Blue - therapeutic use Microbial Viability Photochemotherapy - methods Photodynamic therapy Photosensitizing Agents - therapeutic use Quantitative PCR Real-Time Polymerase Chain Reaction Streptococcus mutans Streptococcus mutans - drug effects
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Beschreibung
Zusammenfassung:	Highlights • We evaluated the antimicrobial effect of PDT in carious lesions in vivo. • We used methylene blue at 100 mg/L associated with a halogen light source. • Viable bacteria were significantly reduced in all of the agar plates after PDT. • The number of Streptococcus mutans DNA copies were not affected after the treatment.
ISSN:	1572-1000 1873-1597
DOI:	10.1016/j.pdpdt.2015.06.003