A method for flow cytometric analysis of Candida albicans DNA

A method was developed by which to simply and easily study the DNA synthetic cycle of Candida albicans. This method employs a standard flow cytometer and the fluorescent DNA binding stain, 4,6-diamidino-2-phenylindole (DAPI). Flow-cytometric analysis by this method resulted in sharp peaks of intense...

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Veröffentlicht in:	Journal of microbiological methods 1998-08, Vol.33 (3), p.191-196
Hauptverfasser:	Stöver, A.G, Witek-Janusek, L, Mathews, H.L
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Candida albicans Cell cycle analysis Flow cytometry Fundamental and applied biological sciences. Psychology Microbiology Mycological methods and techniques used in mycology Mycology
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Beschreibung
Zusammenfassung:	A method was developed by which to simply and easily study the DNA synthetic cycle of Candida albicans. This method employs a standard flow cytometer and the fluorescent DNA binding stain, 4,6-diamidino-2-phenylindole (DAPI). Flow-cytometric analysis by this method resulted in sharp peaks of intense DNA staining. This method is a modification of a widely used protocol for cell cycle analysis in Saccharomyces cerevisiae that was found unsuitable for use with C. albicans. The S. cerevisiae procedure employed propidium iodide which stains C. albicans in a non-specific manner. The use of DAPI resulted in precise C. albicans nuclear staining and eliminated non-specific cellular staining.
ISSN:	0167-7012 1872-8359
DOI:	10.1016/S0167-7012(98)00029-3